The Clinical Observation Of Modification Of BaiTouWeng Formula Enema Treatment On Acute Left Ulcerative Colitis And The Influence On The Intestinal Mucosa IL-8and NF-κBmRNA Expression

Objectives:1) To observe the clinical efficacy of modification of BaiTouWeng formula enema treatment of acute left colonic ulcerative colitis;2) To observe the influence of modification of BaiTouWeng formula enema on intestinal mucosa IL-8and NF-κBimRNA expression;3) To discuss the potential mechanism of modification of BaiTouWeng formula enema treatment on ulcerative colitis.Methods:1)34patients, diagnosed as the acute phase of ulcerative colitis and syndrome of dampness-heat accumulation in large intestine according to TCM differentiation. Patients were randomly enrolled into two groups.2)17patients in treatment group, receive modification of BaiTouWeng formula enema treatment; And the other17patients in control group, receive sulfasalazine retention enema treatment. Each course of the treatment was15days, three courses totally.3)Compare the clinical efficacy and the improvement of intestinal mucosa between two groups at end of treatment. Measure the expression of the intestinal mucosa of IL-8by immunohistochemistry (SP). Measure the expression of the intestinal mucosa NF-KBmRNA by RT-PCR.Results:Comprehensive efficacy on syndromes:The total effective rate in the treatment group and control group was94.11%and76.47%respectively, with statistical significance (P<0.05). The decrease in the total syndrome integral comparison before and after treatment both was statistically significant (P<0.01). Before and after the treatment, the improvement of diarrhea, purulent blood, abdominal pain, abdominal distension, tenesmus, poor appetite and fatigue symptoms in both groups were statistically significant. The improvements of diarrhea, purulent blood and abdominal pain symptoms in treatment group were better than in control group. The improvement of diarrhea symptom has statistical significance (P<0.01) and of purulent blood and abdominal pain symptoms have statistical significant (P<0.05). But the improvement of abdominal distension, tenesmus, poor appetite, and fatigue symptom have no statistical significance (P>0.05).Mucosal lesions effect:Before and after the treatment,the comparison about intestinal mucosa microscopically performance in both groups were statistically significant (P<0.01). The intestinal mucosa performance comparison between two groups was statistically significant (P<0.05). IL-8:Before and after the treatment,the comparison about IL-8expression in intestinal mucosa tissue in both groups were statistically significant (P<0.01). The comparison of IL-8expression in intestinal mucosa tissue between two groups has no statistically significant (P>0.05).NF-κ BmRNA:Before and after the treatment,the comparison about NF-κBmRNA expression in intestinal mucosa tissue in both groups were statistically significant (P<0.01). The comparison of NF-κBmRNA in intestinal mucosa tissue between two groups has no statistically significant (P>0.05).Conclusion:The treatment group and control group both can improve diarrhea, purulent blood, abdominal distension, abdominal pain and tenesmus in patients with ulcerative colitis. The improvement in treatment group of the symptoms of diarrhea, purulent blood and abdominal pain is better than in the control group.Treatment group and control group can improve the intestinal mucosa microscopically performance,and the effect of treatment group is better than control group. Treatment group and control group both can reduce intestinal mucosa tissue of IL-8and NF-κ; BmRNA expression. It indicates that the modification of BaiTouWeng enema formula might treat ulcerative colitis by reducing the NF-κBmRNA expression,therefore,decreasing the production and release of IL-8.