Preparation Of Agarose-Gelatin Scaffold Integrated With PLGA/Naringenin Microspheres On Repairing Of Articular Cartilage Injury

Objective:To evaluate the effects of agarose-gelatin scaffold composited with PLGA/naringenin microspheres on chondrocytes by tissue engineering technology in vitro.Methods:The polymer microspheres contains Naringenin of different concentration were prepared by emulsion evaporation-extraction method.These were then mixed with agarose-gelatin solution in order to fabricated the gel scaffolds.The morphology, drug loading, encapsulation efficiency, controlled release of the microspheres and the porosity of the scaffolds were evaluated.Then the chondrocytes of rabbits which isolated and cultured were seeded in agarose-gelatin scaffold and also the gel scaffold composited with PLGA/naringenin microspheres.During the culture, cell adhesion capability of the gel scaffold were observed.Results:The prepared microspheres showed spherical shape with smooth surface and particle diameter between20to600μm.While the scaffolds had highly uniform porous structure which integrated with the microspheres.Drug loading rate of PLGA/naringenin microspheres was low.With the increasing level of naringenin, drug loading rate was increasing while encapsulation efficiency was decreasing.The porosity, cell adhesion rate and cell activity of agarose-gelatin scaffold composited with PLGA/naringenin microspheres were promising.Conclusion:The agarose-gelatin scaffold composited with PLGA/naringenin microspheres could enhance the viability and biological function of chondrocytes.It could be a novel candidate scaffold for cartilage tissue engineering.