The Effects Of Inhibition Of AMPK Activity On Neuron Apoptosis And Expression Of CytC,AIF After Cerebral Ischemia Reperfusion Injury In Mice

Objective:To observe the effects of Compound C, an AMPK inhibition, on neuron apoptosis and expression of CytC,AIF after cerebral ischemia reperfusion injury in mice, to explore the neuroprotection mechanism of inhibiting AMPK activity. Methods:Seventy-two3-month-old male C57BL/6mice were randomly divided into three groups(n=24):sham group, saline group and drug-treated group. Mice models of middle cerebral artery occlusion(MCAO)were established by insertion of a thread through internal carotid artery. AMPK inhibitor Compound C was injected intraperitoneally in the mice ischemia reperfusion therapy group when the thread was inserted, the same volume of saline was given ischemia reperfusion group at the same time.Twenty-four h after ischemia reperfusion,neurological deficits were observed by Longa method; infarct volumes were measured by TTC staining,the pathology variety was observed by HE staining,the expression of CytC and AIF was detected by immunohistochemistry,neuron apoptosis was observed by TUNEL.Result:Twenty-four h after ischemia reperfusion,compared with the saline group,(1)infarct volume and neurological deficits were both decreased in the drug-treated group (P<0.01,P<0.05,respectively),cortical pathological damage were reduced;(2)ischemia reperfusion drug-treated group cortex and hippocampus on neuron apoptosis and expression of CytC and AIF were reduced(P<0.05). Conclusion:Compound C could have neuroprotection effects in cerebral ischemia reperfusion mice by inhibition AMPK activity. Its mechanism may be related to inhibit CytC and AIF expression,reduce neuron apoptosis.