Association Of The P-selectin Gene-2123G/C、-1969G/A And-1817T/C Polymorphism With Coronary Heart Disease

Objective1、To study the P-selectin gene-2123G/C、-1969G/A and-1817T/C singlenucleotide polymorphism among200CHD patients and210age and sexmatched controls in a Chinese population.And to observe the genotype or alleledistributions of P-selectin.2、To observe the association between different types of coronary heartdisease (CHD) and the gene promoter region-2123G/C、-1969G/A and-1817T/C of the P-selectin gene polymorphism.MethodsThis research take the unrelated individuals as the research objects inLiaoning province.Two hundred CHD patients admitted to our hospital fromJune2012to April2013served as a CHD group and210subjects undergoingphysical examination served as a control group in this study. Polymorphism oftheir P-selectin genes-2123G/C,-1969G/A and-1817T/C was detected byPCR-RELP. CHD patients were further divided into angina pectorisgroup,myocardial infarction group and ischemic heart failure group. Differencein polymorphism of P-selectin genes-2123G/C,-1969G/A and-1817T/C wasobserved in different types of CHD. Results1、The P-selectin gene-2123G/C polymorphism were significantly differentbetween CHD group and controls(x2=6.124,P=0.047；x2=7.355, P=0.008). Butthe distributions of P-selectin gene-1817T/C and-1969G/A polymorphism hadno difference between CHD group and controls(P>0.05).2、The P-selectin genes-2123G/C,-1969G/A and-1817T/C polymorphismwas in strong linkage disequilibrium with each other.Major GGT haplotypeaccounted for60.5%and64.5%of these eight haplotypes in both the cases andthe controls, respectively. By haplotype analyses, there were no significantdifferences in the haplotype frequencies of the P-selectin gene between the twostudy groups(P>0.05).3、P-selectin-2123G/C genotypes and alleles were significantly higher inmyocardial infarction group and ischemic heart failure group than in controlgroup(x2=6.473, P=0.039; x2=13.944, P=0.001)，but there were not differencebetween ischemic angina pectoris and controls(P>0.05).The distributions ofP-selectin gene-1817T/C and-1969G/A polymorphism had no differencebetween different types of coronary heart disease and controls (P>0.05).Conclusion1、P-selectin gene-2123G/C polymorphism was associated with CHD，and-2123C allele is an important genetic susceptibility gene for CHD.2、The P-selectin gene-2123G/C polymorphism were significantly differentbetween different types of CHD and controls，so the P-selectin gene-2123G/Cpolymorphism may be associated with the severity of CHD.