The Pro-effect Of Adipose Derived Stem Cell From Normal Breast On The Migration Potential Of Triple Negative Breast Cancer Cell MDA-MB231

BackgroundMesenchymal stem cells derived from bone marrow (BM-MSCs) have recently been described to localize to breast carcinomas and to integrate into breast cancer associated microenvironment and play a key role in promoting the tumor growth, invasion, metastasis and migration. Carcinoma-associated fibroblasts(CAFs) are also considered to contribute to the migration and metastasis of tumor. The origin of this cell type is not conclusively established and remains controversial though recent studies have shown that at least a subset of CAFs in cancer is derived from circulating BM-MSCs. Compared to BM-MSCs, adipose derived stem cells(ADSCs) as tissue resident stem cells are locally adjacent to breast cancers and may interact with tumor cells directly, however, very little is known about the involvement of this resident stem cell in breast cancer progression as well as whether this type of stem cell can transform into CAFs. The impressive regenerative potential of autologous ADSCs, which can be easily collected from the subcutaneous white adipose tissue, has recently prompted the widespread use of lipotransfer procedures for tissue or organ reconstruction after surgical removal of cancer. Nevertheless, there is still lack of robust evidence indicating that ADSCs is safe in cancer patients currently.Objectives1. To isolate and culture ADSCs from normal breast adipose tissue.2. To investigate the effect of ADSCs from normal breast adipose tissue on the migration potential of triple negative breast cancer cell line MDA-MB231.3. To investigate the effect of ADSCs from normal breast adipose tissue treated with TCM on the migration potential of triple negative breast cancer cell line MDA-MB231. MethodsADSCs were isolated from breast adipose tissues of normal individual in cosmetic and reduction mammoplastic surgery. The migration potential of MDA-MB231under the influence of ADSCs(passage6) was evaluated using a Corning transwell. DIO stained MDA-MB231were seeded alone or in co-culture with ADSCs pre-exposed to TCM or with normal ADSCs in the upper chamber. Migrated cells were fixed on the membrane with4%paraformadehyde and counted in five independent view fields at100magnification of each insert.All values are presented as means±standard deviation (SD). Statistical analysis was performed using SPSS16.0software; P<0.05was considered significant.ResultsWe demonstrated that ADSCs promote cancer cell migration in co-culture. We further showed that the migration effect is more pronounced in ADSCs that have been cultured with tumor cell conditioned medium.Discussion1. Tumor migration-promoting effect of ADSCs from normal breast adipose tissue is via direct ADSCs-tumor-contacts or soluble factors secreted by ADSCs.2. ADSCs can transform into CAF like cells to promote cancer cell migration under the influence of tumor.3. Soluble factors in tumor conditioned medium play a central role in inducing the CAF like cells and the secretion of the very soluble factors is independent of the effect of ADSCs.4. Tumor migration-promoting effect of ADSCs from normal breast adipose tissue is through a hormonal-independent pathway.