The Synthesis Of The BFGF-PLGA Microsphere/HAP/PLLA Framework And Its Biocompatibility On The Rat Bone Marrow-derived Mesenchymal Stem Cells

Objective:The research group chose poly(L-lactic acid)(PLLA) and hydroxyapatite (HAP) as the basic materials, adding basic fibroblast growth factor (bFGF) loaded by poly lactic-co-glycolic acid (PLGA) microsphere and synthesized the bFGF-PLGA microsphere/HAP/PLLA framework with many tiny holes. Then the biocompatibility was verified between the framework with many tiny holes and the rat bone marrow mesenchymal stem cells (BMSCs). Finally, we want to develop a ideal biological framework which has superior osteogenesis ability and excellent biocompatibility to repair bone defects.Methods:The bFGF-PLGA microsphere was prepared by the d-ouble emulsion method, then six materials with many tiny holes (PLLA,PLLA+HAP,PLLA+PLGA microsphere,PLLA+HAP+PLGA mi-crosphere,PLLA+HAP+bFGF,PLLA+HAP+bFGF-PLGA microsphere) were synthesized after mixing bFGF-PLGA microsphere, HAP, and PLLA with many tiny holes by relative techniques. The microsphere and materials with many tiny holes had been characterized by the particle size analyzer, the transmission electron microscopy (TEM), the X-ray diffraction (XRD), the fourier transform infrared spectro-meter, the microcomputer differential thermal balance, and the scan-ning electron microscope (SEM). The toxicity grade of these mater-ials and proliferation of seeded BMSCs were analyzed and compar-ed.Results:For bFGF-PLGA microsphere, the average particle size is about250nm, the average drug-loading capacity is26.03%±0.17%, the entrapment percentage is90.65%±2.68%, the morphology is sp-herical and the dispersibility is satisfactory. The framework (bFGF-PLGA microsphere+HAP+PLLA) with many tiny holes is successfu-lly synthesized. The toxicity grade of the bFGF-PLGA microsphere/HAP/PLLA is the first degree,which indicates no or only a minim-a 1cytotoxity according to the expression from United States Pharma-copoeia. Six materials with many tiny holes (PLLA,PLLA+HAP, PLLA+PLGA microsphere,PLLA+HAP+PLGA microsphere,PLLA+HAP+bFGF,PLLA+HAP+bFGF-PLGA microsphere) are all in favor of the proliferationin of BMSCs and the bFGF-PLGA microsphere/HAP/PLLA is the most favourable one.Conclusions:The bFGF-PLGA microsphere/HAP/PLLA frame-work with many tiny holes has been synthesized successfully which has good biocompatibility to the rat BMSCs in vitro.13charts,5tables,38references.