Expression Of VEGF-C And VEGFR-3in The Tissues Of Liver Cancer And Their Significance

Objective:To detect VEGF-C, VEGFR-3expression in primary liver cancer specimens, and the role of liver cancer occurrence, development and lymphatic metastasis.Methods:To collect surgical resection archived specimens of40patients with primary liver cancer in the Hunan Tumor Hospital, including40cases of primary liver cancer specimens and20adjacent tissues, by use of immunohistochemical staining to examine the protein expression level of VEGF-C and VEGFR-3among them; While to collect fresh specimens of liver cancer from20patients and20cases of adjacent tissues, by means of PCR to detect the expression of VEGF-C and VEGFR-3.Results:VEGF-C expression in cancerous tissues and adjacent tissues were72.5%,25.0%, both of which have significant differences (x2=12.796, P<0.05) and VEGF-C had significant relationship with TNM classification (P<0.05).In the positive and negative hilar lymphnodes, protein expression rates were86.4%and55.6%, both of which had significant differences (X2=4.713, P<0.05). VEGFR-3protein expression rate was82.5%, respectively, next to the liver and cancer tissues,40.0%, there were significant differences (x=11.130, P<0.05); protein expression rates in positive and negative hilar lymph nodes were95.5%and66.67%, respectively, there were significant differences between them (x2=5.583, P<0.05); the expressions of VEGF-C and VEGFR-3were positively correlated (γ=0.748, P<0.001). The survival rates of patients of VEGF-C positive expression were significantly higher than the patient of VEGF-C negative expression (x2=4.313, P<0.05), while there was no significant differences in survival rates between the positive VEGFR-3group and negative VEGFR-3group (x2=2.484, P=0.115). VEGF-C mRNA expression of liver cancer specimen was significantly higher than the adjacent tissues (P<0.01), while the differences between the expressions of VEGFR-3mRNA in adjacent tissues and cancer tissues were not statistically significant (P>0.05); In cancerous tissues and adjacent tissues, the expressions of VEGF-C mRNA and VEGFR-3mRNA had significant relationship with TNM classification. While higher degree of TNM classification, there was more expression of VEGF-C mRNA and VEGFR-3mRNA.Conclusion:The expression of VEGF-C and VEGFR-3both increased in primary liver cancer tissues. The expression of VEGF-C and VEGFR-3have significant relationship with TNM classification. The survival rates of patients of VEGF-C positive expression were significantly higher than the patients of VEGF-C negative expression.10Figures,11Tables,33References...