| Objective1. To observe immune injury in mice by different doses of Clenbuterol (CL);2.To evaluate repair effect of Proanthocyanidins (PC) in CL-induced immuneinjury in mice.MethodsThe80Kunming mice were divided into eight groups, male and female inhalf: the solvent control group, L-CL exposure group, M-CL exposure group,H-CL exposure group, PC group, L-CL+PC group, M-CL+PC group, H-CL+PC group. The mice were fed in special breeding room adaptable in oneweek,and then gavage solutions will be gavage into the mice, which werewashed with deionized water as solvent, according to10ml/kg bw volume.Solvent control group received deionized water, once a day six weeks in total;theses3doses of CL exposure group, namely (L, M, H-CL group) respectively,lower (L-CL0.1mg/kg), middle (M-CL1mg/kg), high (H-CL5mg/kg) CLexposure doses will be gavage into mice intragastrically in six weeks; low,medium and high doses of CL together with PC protection group initiallyexposed to low (L-CL0.1mg/kg), medium (M-CL1mg/kg), high (H-CL5mg/kg) CL exposure dose exposure, and then were given doses of PCprotection (100mg/kg bw) respectively the next day. Animals in each groupwere gavage daily9:00to11:00in the experimental stage once a day. Themice in each group are fed per4-8g/d full price of grain, drinking waterfreely. To detecte the NK cell activity, preparation of chicken erythrocytes,peritoneal macrophage phagocytosis, hemolytic plaque assay antibody-forming cells, the MTT assay lymphocyte proliferation, DNFB induceddelayed type hypersensitivity, swelling calculating, and delayed typehypersensitivity, SOD activity and MDA in serum. At the same time, the spleenand thymus dirty coefficients is weighed and the changes of HE staining ofspleen pathological was observed.Results1.The weight of mice in M-CL group and H-CL exposed group wassignificantly higher (P <0.05); the end weight and net weight of mice in M-CL+PC and H-CL+PC were higher than the PC in the control group (P <0.05);net weight of mice in M-CL+PC and H-CL+PC was less than dose group, anthe difference was statistically significant (P <0.05).2.Spleen histopathology section observation result: the control group, PCgroups: splenic tissue morphology is normal; CL exposure groups: smallstructure spleen is damaged, splenic cord, spleen Sort sinus arrhythmia, arterialwall thickening white pulp, red pulp vascular wall thinning, narrow, centralarterial wall thickening, luminal narrowing, decreased lymphocyte counts; viaPC protection group: spleen tissue morphology improvement, red, white pulp isbecoming clear boundaries, the number of slightly increased.3.Compared with the control group, M-CL group and H-CL infected micethymus, spleen organ coefficient decreased significantly reduced thehematological indices, NK cell activity decreased peritoneal macrophagephagocytosis of chicken erythrocytes significantly reduced the number ofantibody-forming cells decreased lymphocyte transformation capacity SI areweakened, infected mice DTH value becomes smaller, SOD activitydecreased,MDA content increased, the differences were statistically significant(P <0.05); M-CL+PC group, changes in H-CL+PC group of indicators weresignificantly improved (P <0.05).Conclusions1. Clenbuterol (CL) can cause damage to the immune function in mice to different degrees, in which the middle and high dose cause more serious damage toits CL.2. Proanthocyanidins (PC) has some repair effects to immune dysfunction inclenbuterol (CL) mice exposed in varying degrees, in which effect is obvious to themiddle and high dose CL repair damage caused by the immune.3. PC-CL induced immune injury mechanism may be oxidative damage. |
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