Safflower Flavonoids Extraction And Purification And In Vitro Activity Against Atherosclerosis Effect Evaluation

Objective:This research safflower main effective component safflower flavonoid s as the research object, investigated the best extraction and purification process w ere tested in vitro against atherosclerosis and efficacy evaluation of activity and for the further study of safflower preparation and provide basis for clinical application. Methods:1. A moresuitable way to measure the content of flavonoid in safflower i sestablished. In this thesis,different extraction methods have beendiscussed. With the target of extraction quality of flavonoid, somesingle-factor tests have been done su ch as extraction solvent and extractiontime. And require the best extraction technol ogy by doing orthogonal test forthe above factors.2. The purification process:Abso rption rate and desorption rate of total flavonoids were taken as indexes,investigate dabsorptionanddesorptionabilityof6different typesmacroporousresintosafflower flavonoi ds, determined AB-8was optimum macroporous resin. Purification conditions of A B-8macroporous resin were optimized.3. Safflower flavonoids in vitro test to estab lish the mice abdominal cavity macrophage derived foam cell model, using the oxi dized low density lipoprotein (ox-LDL) induced mice abdominal cavity macrophage foam cells, cholesterol ester than total cholesterol values greater than50%populat ion of foam cell formation, then add safflower flavonoids including drug group, an d A positive control HSYA group, cultivate48h, ice bath ultrasonication cells wit h total cholesterol (TC), free cholesterol (FC) kits for testing, observe the therapeut ic effects of foam cells, safflower flavonoids for early atherosclerosis (AS) action r esearch to provide A certain basis.Results:1. The results show that the safflower fl avonoids content of0.0104～0.0299mg·mL-1scope inside sex good, r=0.9995, t he average sample recovery rate was104%, RSD was2.82%. A hydroxyl chrysant hemum yellow pigment in5～35ug·mL-1scope inside sex good, r=0.9996, and to determine the best extraction technology for60times water soak extract twice under60℃,100min at a time.2. The best purification process for type AB-8m acroporous resin adsorption of safflower flavonoids conditions for the sample conce ntration is0.9g·mL-1, the sample flow rate of2mL·min-1, the best sample amou nt is5/8BV, static adsorption time for60min, with7BV50%ethanol to1m L·min-1flow rate of elution.3. Middle dosage safflower extract and HSYA groups has obvious inhibitory effect of foam formation (P<0.05), and with the model gro up than with statistical significance.Conclusion:safflower flavonoids extraction techn ology is stable, simple and feasible. And have a certain resistance to early atheroscl erosis role.