The Influence To Urine Cotinine Concentration Of Smokers And Passive Smokers Due To Circadian Rhythm And Diet

Objective:To study how diet and the circadian rhythm affect Cotinine concentration in urine, control the fluctuate of nicotine metabolism may lead people to smoking cessation and gradually get rid of a lot of tobacco dependence.Methods:32pairs of volunteer couples, male smokers smoke4cigarette in1hour, female co-exposure to1hour. Repeat the test in the fasting state. Extract urine sample within1、6hours for the ELISA test to calculate the concentration of urine Cotinine. Results:Concentration of32male urine samples7:00group:757.4±207.8ng/ml、729.1±173.6ng/ml,13:00groups:562.8±162.0ng/ml,458.3±170.8ng/ml,19: OOgroup:684.3+131.5ng/ml,647.8±205.2ng/ml,24:OOgroup:589.6±202.6ng/ml,484.2±143.8ng/ml. Eating behavior involved process can lead to a increased degree of metabolism. In the same fasting circumstances, the circadian rhythm significantly affect urinary Cotinine concentration, metabolism at night is slow than in the morning Concentration of32female passive smokers were45.2±26.1ng/ml,45.8±14.7ng/ml,32.9±19.6ng/ml,31.7±21.4ng/ml,47.4±25.2ng/ml,38.5±21.0ng/ml,40.1±21.8ng/ml,24.2±15.3ng/ml. All the same,we can easily see the positive strengthen affect by circadian rhythm, and the positive affect of fasting state is not as clear as of the active smokers.Conclusion:Nicotine metabolism of active smokers in daytime is at a high level compare to which at night, easily lead to subsequent increase in nicotine craving behavior, could easily lead to addiction and high-dose demand for tobacco. Prandial state can lead to a transient speed up in metabolism and accordingly with a downward fluctuate of Cotinine concentration in plasma. Passive smokers affected by the randial state is still statistically meaningful, however the absolute value does not changed much. While the impact of circadian rhythm is not so clear which is probably due to a rather low cotinine level in their body.