Study On The Noninfectious Mechanism Of Microtus Fortis To Schistosoma Japonicum

Objective:Schistosomiasis caused by schistosome, is a wide spread parasitic zoonosis that causes serious healthy problem to both human and animals.The rearch builded anti-schistosomiasis animal mode of Microtus fortis. Pathology and gene sequences of livers and lungs from Microtus fortis were observed when6、10、15、20、30days after infected with Schistosoma. The expression levels of diffirent genes which is related to growth and development were detected at different time points.Those genes were screened out from different literatures and and the rearch results which was aimed to clarify the natural resistance mechanisms of Microtus fortis to Schistosoma japomcum.Methods:Thirty10-week-old Microtus fortis were randomly divided into six groups and there are five Microtus fortis in each group. The first group was the control group, and the2-6groups were infected with Schistosoma japomcum. The infection dose was2000.Pathology of lungs and livers was observed when6,10,15,20,30days after infected with Schistosoma japonicum.Fresh liver tissues of four10-week-old normal Microtus fortis were taken to extract total RNA that was used to sequence the cDNA by SMART, and the biological level, the cytological level, molecular level of genes distribution were summarized.Lysozyme gene (LYZ), MHC class Ⅱ molecules (RT1-DB1) gene, CD74gene (CD74), complement1q gene (Clqa), thyroid receptor a gene (thra) were screened out through combining the sequencing results with literatures, whose expression levels were detected when6,10,15,20,30days after Microtus fortis infected with Schistosoma japonicum.Results:Heavy bleeding points, pulmonary and slightly increased were Observed in lungs 6-10days after Microtus fortis infected with Schistosoma, the bleeding points disappear after15days,some scattered white spots were observed in the surface of the lives between10-20days after infected with Schistosoma, the liver body was lightly increasesand, its color was darker and back to normal30days after. Serious pathological damage, local bleeding, telangiectasia, wall thickening, bronchial and perivascular and a large number of inflammatory cells in lungs was detected by microscope at the first6-10days,and recovered after20days; sinusoidal height, a large number of eosinophils and a small amount of neutrophils around the parasites in liver6-20days after,and recovered after30days.The cDNA sequences of Microtus fortis revealed the biological level, the cytological level, the molecular level functional and Lyz, RTl-Dbl, Cd74, C1qa, Thra, Igfl gene sequences were founded fom that.Microtus fortis lung infections Schistosoma Lyz,RT1-DB1, CD74were gene significantly up-regulated in lungs at the first6-10days after Microtus fortis infected with Schistosoma (P<0.01),and began to return to normal expression levels from the15th day; Lyz, RT1-Db1, Cd74were significantly upregulated in livers in10-15days (P<0.01) and began to return to normal expression levels from20th day. C1qa gene was significantly increased in lungs in6-10days (P<0.01) nd began to return to normal expression levels from the15th day while had no expression change in livers. Thra gene was significantly reduced in lungs in10-15days (P<0.01), gradually restored to normal levels from20th day while had no expression change in livers; IGF1had no expression change in lungs while significantly reduced in10-15days (P <0.07),and gradually restored to normal levels from20th day.