The Effect Of Treadmill Running To The Spatial Memony And The Expression Of Ku Protein And Cell Cycle Protein In Rats After Global Cerebral Ischemia Induce By Hyperlipidemia

Objective To investigate the effect of treadmill running to Ku protein and cell cycleprotein in rats after Global cerebral ischemia induce by hyperlipidemia.Methods120healthy male wister rats were randomly divided into: control group(n=40),model group(n=40), experiment group(n=40). each group into3h,6h,24h,48h time point,each group at each time point of10rats. hyperlipidemia was formed by high-fat diet for6weeks, experiment group in injury before15days treadmill running20meter everyminute every day. Global cerebral ischemia model was formed by improved four-vesselocclusion as described according to the description of Pulsinelli’s method. By using theMorris water-maze test Visual-spatial Memory of rats at24h and48h; method withHE(hematoxylin-eosin staining)to observe the changes of Global cerebral ischemia in therat brain hippocampus neurons form at each time point; using immunohistochemicalstaining and Western blot protein(western-blotting)detected in rat brain tissue Ku70、Ku80、CyclinA and CyclinE protein expression at each time point. Repeated applicationSPSS13.0statistical analysis software obtained experimental data to design one-wayANOVA(P<0.05), a statistically significant difference.Results1the Morris water-maze test Visual-spatial Memory of rats: Compared withcontrol group, in model group, the escape latency of rats was prolong; the incidence(n/90S) of crossing the area where the platform had been located on previous trials wasreduced (P<0.05). Compared with model group, in experiment group, the escape latencyof rats was significantly reduce;the incidence (n/90S) of crossing the area where theplatform had been located on previous trials was increase (P<0.05).2Morphologicalobservations of HE staining: in control group, the neuronal cells significantly changed,part of the cells arranged loosely, abnormal shape. in model group, the change at3h;appears bubble around the cells, the cell nucleus shrinkage at6h; part of the nucleolusdisappears, coloring deep, interstitial edema phenomenon at24h; the cell nucleussignificantly shrinkage, coloring deep, the nucleolus disappearsat48h. experiment groupshowed more remnants of the nerve cells, neuronal morphology is relatively normal,3hless time edema,48h neuronal cell pycnosis and vacuolation, but their number was small;The number of necrotic neurons was significantly less than the model group and low and medium dose group.3Result of Ku70immunohistochemistry and Western blot:Immunohistochemistry: Ku70was stained brownish yellow, located in the cytoplasmicand mainly expressed in neurons cells. Compared with control group, in model group,thepositive cells was reduce (P<0.05). Compared with model group, in experiment group,the positive cells was significantly increased (P<0.05). Western blot: Compared withcontrol group, in model group,the positive cells was reduce (P<0.05). Compared withmodel group, in experiment group, the positive cells was significantly increased (P<0.05).4Result of Ku80immunohistochemistry and Western blot: Immunohistochemistry: Ku80was stained brownish yellow, located in the cytoplasmic and mainly expressed in neuronscells. Compared with control group, in model group, the positive cells was reduce(P<0.05). Compared with model group, in experiment group, the positive cells wassignificantly increased (P<0.05). Western blot: Compared with control group, in modelgroup, the positive cells was reduce (P<0.05). Compared with model group, inexperiment group, the positive cells was significantly increased (P<0.05).5Result ofCyclinA immunohistochemistry and Western blot: Immunohistochemistry: CyclinA wasstained brownish yellow, located in the cytoplasmic and mainly expressed in neuronscells. Compared with control group, in model group, the positive cells was increased(P<0.05). Compared with model group, in experiment group, the positive cells wassignificantly reduce (P<0.05). Western blot: Compared with control group, in modelgroup, the positive cells was increased (P<0.05). Compared with model group, inexperiment group, the positive cells was significantly reduce (P<0.05).6Result ofCyclinE immunohistochemistry and Western blot: Immunohistochemistry: CyclinE wasstained brownish yellow, located in the cytoplasmic and mainly expressed in neuronscells. Compared with control group, in model group, the positive cells was reduce(P<0.05). Compared with model group, in experiment group, the positive cells wassignificantly increased (P<0.05). Western blot: Compared with control group, in modelgroup, the positive cells was reduce (P<0.05). Compared with model group, inexperiment group, the positive cells was significantly increased (P<0.05).Conclusion1treadmill running improve the function of spatial Memory after Globalcerebral ischemia.2treadmill running increase the expression of Ku protein after Globalcerebral ischemia, and regulation CyclinA and CyclinE, has protective effect on cerebralbrain tissue.