| Background:Stroke is the third leading cause of disability and the world' s most deadly disease. Stroke is divided into ischemic and hemorrhagic types. 80% of cases are due to ischemia and the other 20% are from hemorrhage. The main type is "ischemic stroke, " the high incidence affects major area. Although all current medicine is power less to help dead necrotic tissue at the center of the ischemia, if treatment is applied in an effective time, further damage to penumbra ischemic areas can be prevented and help promote survival of tissue. Thus reducing mortalityrate, disability rate, and promoting quality of life of patients. Prompt treatment of ischemic stroke is extremely important."The brain is the sea of marrow; its to paccess is at its cover (BaiHuiGV20)" . The "DU meridian" and the brain have a very close relationship. The "DU meridian" directly communicates with the brain. "BaiHui" (GV20) and "DaZhui" (GV14) are both acupoints on the "DU meridian" . "BaiHui", also known as "the five unions of the three yang" , is the gathering place of the hundred pulses. "DaZhui" is known as "the union of the three yang and DU meridian, " or the association of the yang from the legs and arms. Chinese medicine calls the brain "the sea of marrow, " it' s qi and blood flow up to the cover (BaiHui) , and flowdown to "FengFu" (GV16). Therefore, BaiHui and DaZhui are especially effective in treating cranial disorders.In the records of classical Chinese Medicine, BaiHui and DaZhui are very commonly used acupoints for emergencies, particularly stroke. No matter, if the treatment is for prevention, acute phase meridian or viscera treatment, remission or residual phase, these acupoints are the most common. It is very worthwhile to deeply in vestigate the use of these points during acute phase stroke with viscera and body coma. Therefore, this research will evaluate the use of electro-acupuncture of BaiHui and DaZhui in preventing damage to the brain during acute phase stroke. Objective:In this research, the global cerebral ischemic rat models were established by internationally acknowledged means of occlusion of four-vessel occlusion (4-VO). All rats were divided into four groups randomly: blank control group , sham operation group, model group and electro-acupuncture group. Baihui and Dazhui were selected as acupuncture points. After successful modeling, the rats in electro-acupuncture group were electro-acupunctured on BaiHui and Dazhui for five days, and orther groups received no treatment. After the experiment, the expression of TNF- a , IL-6, Glu and Asp in the cerebral cortex were measured, and the results were analysed statistically. Results:1. Electro-acupuncturing on Baihui and Dazhui significantly reduced the volume of cerebral infarction.2. After global cerebral ischemia-reperfusion, the positive stain index of Cytokine TNF- a and IL-6 in cerebral cortex increased, but electro-acupuncturing on Baihui and Dazhui could decrease the positive stain index.3. After global cerebral ischemia-reperfusion, excitatory amino acid Glu and Asp contents in pallium increased significantly, but electro-acupuncturing on Baihui and Dazhui could decrease these contents significantly. Concusion:Electro-acupuncturing on Baihui and Dazhui can control the global cerebral ischemia-reperfusion injury effectively through immune regulation mechanism of inflammatory reaction after cerebral ischemia and decreasing excitatory amino acid Glu and Asp contents in pallium. That is to say , it can protect neurons and control cerebral ischemic injure through decreasing the positive stain index of Cytokine TNF-a and IL-6 in cerebral cortex and inducing Glu and Asp content.
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