Fructus SchB The Protective Effect And Mechanism Research Of UVA

Objectives1To investigate the fruit of SchB element to long-wave ultraviolet(UVA) induced skin cutin form protective effect and its possible mechanisms ofcellular damage.2Whether the research fruit of Chinese magnoliavine b have thefunction of the uv radiation induced acute injury of light, and to explore itsmechanism.Methods1Will have to extend HaCat cell cultivation bottles in5%CO2incubator,10%FBS in sugar DMEM culture, in every day. Experimental set in thecontrol group (without UVA irradiation), model group (UVA exposure dose for1,5,10,15J/cm2) and ShcB1~4groups (UVA irradiation after joining ShcB0.1respectively,0.01,0.001,0.0001μmol/L). UVA is320~400nm wavelength,irradiation distance is1cm. Pour to each group of cell culture, PBS wash twice, thenadd the right amount of cold PBS coverage, to join the serum-free culture continuesto develop, after irradiation by detecting the cell survival rate to determinethe properirradiation intensity. With appropriate intensity of UVA processing HaCat cells, givedifferent concentrations of SchB UVA HaCat cells after injury.2K-unming micewere randomly divided into four groups, each group of10. Outside the normalcontrol group, model group, the matrix group,0.3%of fructus schisandrae b group,in addition to the normal control group, the rest of the group are exposure to10J/cm2UVA, with HE staining to observe the pathological changes of each group ofimmunohistochemical observation Ⅰ, Ⅲ collagen type; Bax,the change of theBCL-2.Results1UVA dose experiment to determine the control group and1,5,10,15J/cm2model group HaCat cell survival, respectively (100.00±6.31)%,(98.10±5.78)%,(75.05±8.64)%,(66.10±5.61)%and (53.71±2.59)%, thedifference was statistically significant (P <0.01). With the increase of UVAirradiation dose, the HaCat cell survival rate gradually reduced, when it was foundthat the cell survival rate lower than70%, when it is used to add SchB damagedalmost NO longer HaCat cell proliferation, gsh-px, SOD and LDH, almost NOdifference, does not conform to the actual situation, so choose5J/cm2as experimental exposure dose.2Different concentration SchB on HaCat cells afterinjury of UVA protection, SchB3group cell survival rate is highest, but with thedecreasing concentration of SchB, reduce the drug’s protection.3Differentconcentrations SchB on HaCat cell gsh-px, SOD and LDH, NO content comparedwith control group, the influence of model group of gsh-px, SOD activity decreased,LDH, NO content increased (P <0.05). Compared with model group, SchB1~4groups can improve the activity of SOD, reduce the content of LDH, NO, exceptSchB4group SchB1~3groups can improve the activity of gsh-px.4The fruit ofChinese magnoliavine b element can inhibit the mice skin damage caused by UVAirradiation.5The experiment shows that the Bcl-2protein expression of UVAexperimental mice significantly reduced; The expression of Bax protein increasedsignificantly; Collagen I, Collagen III Collagen protein expression decreasedsignificantly. Fructus schisandrae b element can increase Collagen I, III Collagenand Collagen the Bcl-2protein expression of Bax protein expression (P <0.01),with statistical significance.Conclusions1Through the study in vivo and in vitro, fructus schisandrae belement through the antioxidant activity of suppressing UVA radiation inflammation,oxidative stress and cell apoptosis.2By reducing the degradation of type I and typeIII collagen, so as to achieve uv protection.3The fruit of Chinese magnoliavine belement can be adjusted through the light aging mice skin tissue and promoteapoptosis gene Bax protein expression, increase the expression of apoptosisinhibiting gene Bcl-2protein, reduce skin cells apoptosis, and delay the ageing ofthe skin is light.4The fruit of Chinese magnoliavine b element can effectively lessafter UVA irradiation, the SD rat skin wrinkles, improve skin rough skin and brittle.5above all fructus SchB element has a clear light damage resistance and lightresistance aging effect. Promising to develop effective uv protection product andantioxidants, which can be widely applied to cosmetics and uv protective drugs, hasa high research value.