Effects Of Candidate Tumor Suppressor Gene OPCML On Proliferation, Apoptosis, Invasion And Migration Of Human Breast Cancer MDA-MB-231Cells

Objective To investigate the effects of OPCML expression on proliferation, apoptosis,invasion and migration of human breast cancer MDA-MB-231cells.Methods Chemically synthesized OPCML was transfected into MDA-MB-231cells ofhuman breast cancer by Lipofectamine2000. The proliferation of MB-231cells was determinedby Colony formation assay in vitro and tumorigenicity studies in vivo, the cell apoptosis wasdetected by Flow cytometry, and the invasion and migration ability was detected by transwelland wound healing assay.Results OPCML-pcDNA3.1and pcDNA3.1was successfully transfected into MB-231cells. Colony formation assay results showed that the multiplication capacity depressed afterOPCML transfect human breast cancer cell MDA-MB-231successfully. The MDA-MB-231cell coloning efficiency of the OPCML transfection group and pcDNA3.1group was (28.67±2.52)%，(47.67±4.51)%，(P<0.05). The OPCML group，the mean tumor volume was (63.77±27.07) mm3，compared to the mean volume of the vector tumor，which was (155.14±92.29)mm3,(P<0.05)；In the OPCML group，the apoptosis rate of MB-231cells wassignificantly up-regulated more than that in the pcDNA3.1group [(21.99±1.14)%vs(15.08±1.66)%，P<0.05). The migration of MB-231cells in the OPCML group (30.67±4.04)μmwas inhibited significantly as compared with the empty vector group （72.33±4.51）μm anduntransfected group [(66.12±6.56)μm，P <0.05), and the invasion of MB-231cells in theOPCML group was suppressed significantly as compared with the empty vector group and theuntrensfected group [(35.33±3.51) vs (63.26±4.36)、(61.37±8.21)]，P<0.05.Conclusion The expression of OPCML can inhibite the proliferation, migration and invation, and increase apoptosis of human breast cancer MDA-MB-231cell. OPCML was atumor suppressor gene, can be regarded as a candidate gene for breast cancer gene therapy.