Study On Combined Optical Methods For Detection Of Biomarkers In Blood

Blood test represents the most common technique for patient treatment, anddetection of blood components plays a critical role in the diagnosis of diseases.Among diverse analysis techniques, the optical detection technique has become one ofthe optimal techniques for blood tests due to its significant advantages such as beingfast and convenient. Different types of optical detection techniques, e.g. luminescenceimmunoassay technology, Raman spectroscopy, etc. reflects different the informationof blood component with different concentration respectively, which make thecomplementarity of information possible. However, false detection and detectionincompleteness might occur if a sole detection technique is applied because thecomposition of blood is extremely complex. In this regard, it is highly necessary tointegrate different types of detection technologies to improve the accuracy andreliability of the detection of blood components, making mutual correction andcomplementary strengths available.In this work, the optical detection technique is used for research and the immuneemission analysis and Raman spectroscopy are integrated with each other in order todetect a wide range of blood components. Some investigations are made on themechanism of the generation and analysis of the optical signal, which is aimed atmaking the advantages and disadvantages of different technologies clear. The wholestudy is listed as follows.1. Reviewing of present situation of domestic and foreign, as to the demandbackground for blood components testing, a detailed analysis of theimportance of blood components testing was made. And summarized presentsituation of the optical detection of blood components and then focused ontwo typical optical detection methods and their advantages.2. Detail analyses of the principle of Raman spectroscopy detection techniquesand luminescent oxygen channeling immunoassay were made respectivelyand comparison were carried out among their advantages and disadvantages.We have illustrated the necessity and significance in theory that these twomeasurement methods must be linked with chemical reagents to realize theenhancement of signal.3. As to the detection of trace blood components, the detection performance of tumor markers were verified by experiments on homemade homogeneousimmunoassay instruments proving the technique s advantages in detectionaccuracy and detection sensitivity, thus it is expected be a mean of screeningearly cancer.4. For conventional blood components testing, conventional serum componentswere detected using Raman measurement platform which was built inlaboratory. Experiments showed that proteins markers were expected to bedetected directly applying Raman spectroscopy. In addition, we described themethods applying the least squares model to improve the detection accuracyof spectral.In this research, a combined optical detection method was proposed byintegrating luminescence immunoassay technology with Raman spectroscopy toanalyze the components within different range of concentration in blood, andcombined with the multivariate modeling technique, initial integrated diagnosis usingintegrating luminescence immunoassay technology with Raman spectroscopy wasachieved, which resulted in mutual correction and complementary strengths. Theresult indicates that this technique has the potential of multi-parameter detection inblood biomarkers.