| BackgroundPancreatic cancer is a kind of malignant tumor that occult onset and progress rapidly. Its curative effect and prognosis is poor. With the development of economy and society, its incidence increased year by year. At present, pancreatic cancer is the fourth influence factor that causes patients with malignant tumor to death.It is the second influence factor that causes patients with digestive system’s malignant tumor to death. This ranking is only behind colorectal cancer. This disease is sent more at 40 years of age or older, more men than women to see. Pancreatic cancer is very difficult to find with existing examination, because it’s easy to invade neighboring important vascular, nerve, lymph nodes nearby as to its high malignant degree. Pancreatic cancer incidences hidden and develops fast. When most of the patients go to a doctor, they have been in the advanced stage or have been widespread metastasis.Then they have lost the operation chance. Pancreatic cancer is not sensitive to radiotherapy and chemotherapy.Its resection rate by operation is very low(only 15-30%). Metastasis and recurrence rate is high,and the prognosis is poor.So therapeutic effect of routine treatments for pancreatic cancer is very poor. 90% of the patients died within one year and the 5 year survival rate is only about 1.5%. Due to the lack of early diagnostic method and treatment method after diagnosis, pancreatic cancer has been a threat to human health. It has become the urgent problems to be solved to actively study and explore new methods of diagnosis and treatment to inhibition the invasion and metastasis of pancreatic cancer and to improve the prognosis of pancreatic cancer. The pathological characteristics of pancreatic cancer is mainly attributed to its high metastatic and invasive. There is important significance to detection the change of gene and the expression of tumour markers in the process of pancreatic cancer’s occurrence and development.In the process of malignant tumor’s occurrence and development, cancer cells’ over division and proliferation has been the most notable feature. Division and proliferation of all cells need activation of protein synthesis system, and then a large number of proteins that can provide the necessary material foundation for the division and proliferation of cells are produced.So, activation of protein synthesis system can induce the synthesis of a large number of proteins within the cell. This provides an essential condition for the division and proliferation of tumor cells. The current study shows that mammlain target of rapamycin(m TOR) plays the crucial role of regulation in the process of cell’s division and proliferation,and eukaryotic initiation factor 4E(e IF4E) in m TOR’s downstream is closely related with tumor’s occurrence and development. Objectivestudy the expression and significance of m TOR and e IF4 E factors in primary pancreatic carcinoma,and analysis The correlation between them to study m TOR and e IF4E’s role and clinical significance in the occurrence and development process of primary pancreatic cancer. MethodsCollect the clinical data of 72 cases of pancreatic cancer who accepted operation treatment in the First Affiliated Hospital of Zhengzhou University from September 2012 to September 2013. Among 51 male patients, aged 35-75 years old, average age was 51 years old. Among 21 female patients, age 37-73 years old, mean age was 51 years old. All patients were first treated with operation, and they hadn’t accepted any form of radiotherapy,chemotherapy. Pathological examination of all cases were confirmed as pancreatic ductal adenocarcinoma. In immunohistochemistry, the method wo used is SP(streptavidin peroxdase conjugated method), In order to determine the expression of m TOR and e IF4 E in pancreatic ductal adenocarcinoma, pancreatic benign tumors and normal pancreas. For all the experimental data, We use SPSS 17 statistical software for statistical analysis. We compare the positive rate of m TOR and e IF4 E in pancreatic ductal adenocarcinoma, pancreatic benign tumors and normal pancreatic tissues by Fisher test and χ2 test. Analysis the correlation between m TOR and e IF4 E in pancreatic ductal adenocarcinoma by Spearman rank correlation analysis. Results1. m TOR’s expression: m TOR had the expression in normal pancreatic tissue, benign pancreatic tumors and pancreatic ductal adenocarcinoma. The difference between benign pancreatic tumors and pancreatic ductal adenocarcinoma was statistically significant( χ2 =41.57, P<0.05).The difference between normal pancreatic tissue and pancreatic ductal adenocarcinoma was statistically significant(χ2=28.73,P<0.05).There was no statistical significance in the difference between normal pancreatic tissue and benign pancreatic tumors(χ2=0.02,P>0.05).2.e IF4E’s expression: m TOR had the expression in normal pancreatic tissue, benign pancreatic tumors and pancreatic ductal adenocarcinoma. The difference between benign pancreatic tumors and pancreatic ductal adenocarcinoma was statistically significant(χ2 =30.51,P<0.05). The difference between pancreatic ductal adenocarcinoma and normal pancreatic tissue was statistically significant(χ2=20.88,P<0.05). There was no statistical significance in the difference between benign pancreatic tumors and normal pancreatic tissue(χ2=0.04,P>0.05). 3. The correlation between m TOR and e IF4 E in pancreatic ductal adenocarcinoma tissues: In pancreatic ductal adenocarcinoma tissue m TOR and e IF4 E expression existed correlation, and appear to be a positive correlation. Conclusion1.The expression of m TOR protein in pancreatic ductal adenocarcinoma was higher than that in benign pancreatic tumor tissue and normal pancreatic tissue. Expression of m TOR in pancreatic ductal adenocarcinoma tissue was positively related to whether there was lymph node metastasis, clinical stage and pathological grade. It is suggested that m TOR protein may be related to pancreatic cancer’s invasion and metastasis.2. The expression of e IF4 E protein in pancreatic ductal adenocarcinoma was higher than that in benign pancreatic tumor tissue and normal pancreatic tissue. Expression of m TOR in pancreatic ductal adenocarcinoma tissue was positively related to whether there was lymph node metastasis, clinical stage and pathological grade. It is suggested that e IF4 E protein may be related to pancreatic cancer’s invasion and metastasis.3.The expression between m TOR and e IF4 E in pancreatic ductal adenocarcinoma tissue was positively correlated. This suggests that the two may have synergistic effect, so they could control the tumor’s invasion and metastasis together. |
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