| Objective: Iron regulatory proteins(hepcidin), a peptide hormone primarily expressed in liver, has direct bactericidal and bacteriostatic activity as antimicrobial peptides, can inhibit the growth and reproduction of pathogen which is uptake through iron metabolic pathway and can adjust the balance of iron absorption, utilization and storage and iron homeostasis as well. Recent research demonstrated that cancer, inflammation, infection and immune dysfunction could activate immune cells. Then TNF-α, IL-6, IL-1, IL-6 and IL-10 were released increasingly and higher expression of hepcidin induced by activating JAK2 / STAT3 pathway in the liver. It was also reported that elevated EPO in anemia could inhibit the level of hepcidin. Liver cancer anemia patients, who belonged to chronic inflammatory anemia(ACD), were commonly associated with inflammation. However, the relationship between anemia and the mechanisms of occurrence and development are still unclear in inflammation-related liver cancer. The purpose of this study is to observe the expression of hepcidin, EPO, IL-6, CRP and iron parameters in liver cancer anemia patients by using ELISA and spectrophotometric methods and explore the correlation between hepcidin and EPO, inflammatory cytokines( IL-6, CRP). Further to provides a theoretical basis for claritying the mechanism of liver cancer anemia and finally find new perspectives for effective treatment.Methods: The experiments were divided into two groups: control group(30 cases), liver cancer anemia group(40 cases). All blood samples were collected after 8 h of fasting. Two ml blood was mixed thoroughly with EDTA-K2 anticoagulant in vacuum tube. The ratio of blood and anticoagulant agents is 1:9. Serum was obtained by centrifuge 5 minutes with 5ml blood(3000g / min). Detecting blood routine by LH750.Hepcidin-25, erythropoietin(EPO), serum ferritin(SF) and serum transferrin(TRF) were detected by ELISA method. Serum iron(SI) and total iron binding capacity(TIBC) by the colorimetric method using Spectrophotometric, C- reactive protein(CRP) by immune turbidity method. All data were analysed to explore expression of serum hepcidin in liver cancer anemia patients and correlation between hepcidin and inflammatory factors. Date were analysed by Statistical software, P <0.05 was considered significant statistically.Results:1 Compared with control group, the expression of hepcidin was increased in liver cancer anemia group, but there was no significant difference(P > 0.05).2 Compared with control group, EPO value was increased significantly in liver cancer anemia group(P < 0.001).3 Compared with control group, the level of serum IL-6 was significantly increased in liver cancer anemia group(P < 0.001), and the level of CRP was also significantly increased(P < 0.001).4 Compared with control group, the levels of serum ferritin and transferrin were increased significantly in liver cancer anemia group(P <0.001), and serum iron was decreased significantly(P < 0.001).5 There were no significant correlation between hepcidin and Hb, CRP, IL-6, SF, TRF, TIBC, SI, EPO. Correlation coefficients respectively were Hb(r =-0.277, P = 0.084), CRP(r = 0.126, P = 0.439), IL-6(r = 0.169, P = 0.296), SF(r = 0.263, P = 0.101), TRF(r = 0.248, P = 0.123), TIBC(r = 0.076, P = 0.643), SI(r =-0.211, P = 0.190) and EPO(r = 0.275, P = 0.086).Conclusions: The level of serum EPO and IL-6 were increased significantly in liver cancer anemia patients. There was no significant difference of hepcidin expression between two groups, which may be related to the inhibition of EPO. There was no significant correlation between hepcidin and the indicators of iron parameters. |
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