Effect Of Arabinoxylans-Rich Extract Of Wheat On Intestinal Health In Mice

ObjectiveTo determin the changes of the nucleic acid and morphology in intestinal as well as the microorganism in colic contents, we investigate the Arabinoxylans dosage range which affect the function of intestinal. Meanwhile, study the impact on intestinal morphology, gut microflora and intestinal barrier function,in order to provide experiments basis for explore the mechanism of action which Arabinoxylans-rich extract of wheat (AXEW)make to intestinal function.Methods1. Forty adult KM mice were randomly divided into four groups:control group (CN), low-dose group (0.4 g/(kg·bw·d) AXEW, LD), middle-dose group (0.8 g/(kg·bw·d) AXEW, MD), high-dose group (1.6 g/(kg·bw·d) AXEW, HD). AXEW was given once a day through intragastric administration for 2 w. At the end of the experiment, intragastric D-xylose and make orbita blood collection after aether anesthesia lhour later, using phloroglucinol method to detect the serum D-xylose2.The animals were cacrificed at 2 weeks after operation.the jejunum were taken out to make pathological section and determine the nucleic acid by Ethidium bromide method.take the colonic contents under aseptic conditions,determine its microflora content were determined by plate counting of bacteria method, and short-chain fatty acids were determined by Gas chromatography.Results1. The different AXWE intragastric dose in KM mice, in low and middle groups,the contents of DNA and RNA were significantly increased (P＜0.05),meanwhile,the intestinal villus and small intestinal gland were significantly increased, the high dose group intestinal villus decreased and small intestinal gland increased in contrast.the contents of D-xylose on serum were significantly decreased in low group (P＜0.05)2. Compared with control, The contents of bifidobacterium and lactobacillus in colonic contents were significantly increased, however, Escherichia coli and enterococcus faecalis were significantly decreased, The short-chain fatty acids (SCFAs) significantly increased, while pH was significantly decreased. Compared with control group, all the differences are statistically significant (P＜0.05).ConclusionThis study shown that after different AXEW intragastric dose 2 w, an impact on mice intestinal function were observed.In our result, at the low and middle groups,the contents of DNA and RNA were significantly increased,in morphology,we found that both intestinal villus and small intestinal gland were increased,therefore,we consided that low and middle group AXEW promote the proliferation of intestine cell. The possible mechanisms is the hydrolysis product of arabinoxylans nutrition stimulu action.The high dose group had no difference in intestinal nucleic acid, but intestinal villus were decreased, The possible reason is that the high viscosity of the AXEW. Serum D-xylose were decreased in low dose group,means the intestinal barrier function has been improved, The possible mechanisms is that AXEW were wassociated with intestinal mucosa.A dose of AXEW intragastric can increase the bifidobacterium and lactobacillus as well as the short-chain fatty acids (SCFAs)in colonic contents, however, Escherichia coli and Enterococcus faecalis were significantly decreased, significantly increased. The reason might be bring about with prebiotics function of arabinoxylans.