Effect Of Vitamin E On Neurogenesis And Expression P-Creb In Hippocampus Of Manganese Poisoning Mice

Objective:To study the effect of vitamin E on neurogenesis and expression of the hippocampus p-CREB in manganese poisoning mice.Methods:Good health Kunming mice,75 male, were divided into five groups,15 for each group compose of the vitamin E group (EG), the control group (CG), the manganism add vitamin E group (MEG), the manganism add Probucol group (MPG) and the manganism group (MG). Using manganese chloride to intraperitoneal injection processed the maganism mice, the learning and memory capacity of the mouse was observed by Morris water maze, neurogenesis and the expression of p-CREB was tested by the Immunohistochemistry methods.Results:Morris water maze navigation experiment:The escape latency was significantly shorter and the times of passing through platform in space probe test higher in CG, EG, MEG and MPG than those in MG Behavioral testing finished, then brains were removed and analyzed the dentate gyrus (DG) and CA1 of the hippocampus were analyzed for GFAP, DCX and p-CREB immunohistochemistry. Manganese mice exhibited decreased motor activity and cognitive impairment compared with normal controls. The MG group reversibly affects neurogenesis targeting difference in the dentate gyrus. The number of p-CREB positive neurons was decreased in the DQ CA1 of manganese mice. The present study showed that chronic administration of vitamin E ameliorates memory and study in manganese mice and revealed that neurogenesis and p-CREB producing neurons.Conclusion:These results provide insights into molecular mechanism manganese neurotoxic and suggest the potential use of vitamin E by improved neurogenesis and p-CREB expression as an adjuvant therapy for aberrations memory, study and the prevention and treatment of manganese conditions.