The Effect Of Vitamin E On Learning And Memory And Nscs Of Manganese Exposure Mice

Object: Production manganese poisoning animal models, adopt the methods of neurobehavioral and immunohistochemical studying the effects of manganese exposure in mice hippocampal nerve cells and the intervention effect of vitamin E on which, and to explore the mechanism, provide a reference for the mechanism and intervention of manganese poisoning.Methods: 1. Animals and grouping: Adult male mice of clean grade 75, weighing 18-22 g(provided by the Experimental Animal Center of Guangxi Medical University). The 75 mice were divided into five groups control principles randomly, namely the control group(CG), the vitamin E group(VG), manganese poisoning group(MG), manganese and vitamin E group(MVG), manganese and probucol group(MPG), each group has 20. 2. Production of manganese poisoning mice: Respectively, manganese poisoning group, manganese and vitamin E group, manganese and probucol group three groups of mice by intraperitoneal injection ofmanganese chloride(Mncl2), at a dose of 20 mg / kg, the remaining two groups of mice were injected with saline volume. Injection once every afternoon, continuous injection of 4 weeks. 3. Drug orally: Be respectively the vitamin E group, manganese and vitamin E group intervention vitamin E drug orally at a dose of 100 mg / kg, in the control group and the group of manganese poisoning, such as the volume of olive oil to be fed, manganese and probucol group were given probucol drug orally intervention at dose of 500 mg / kg, the mice gavage once a day in the afternoon, for 4 weeks. Each group of mice rearing in the same environment, free diet. 4. Neurological behavior detecting: After 4 weeks, by Morris water maze detecting the escape latency and the number of cross platform, thereby determining the ability of learning and memory in mice in each group. 5. Immunohistochemistry: Using Immunohistochemical method detecting mice in each group the expression of caspase-3 in hippocampal CA1 and DG regions, the expression of cytochrome C in hippocampal CA1 and DG regions and the expression of nestin in dentate gyrus granule area. Calculated immunohistochemistry images positive cells, using statistical software Graph Pad Prism 5 and spss16.0 analyzing the data of Morris water maze and immunohistochemical results.Result: 1. Morris water maze test results: Water maze test is divided into place navigation and space exploration experiments, in placenavigation test, compared with the control group, the escape latency of manganese poisoning mice was prolonged, the difference was statistically significant(P <0.05); Compared with manganese poisoning group, the escape latency of manganese and vitamin E group and manganese and probucol group were significantly shorter, and the difference was statistically significant(P <0.05); The escape latency of manganese and vitamin E group and manganese and probucol group compared with no significant difference(P> 0.05); The escape latency in the control group and the vitamin E group were compared, the difference was not statistically significant(P> 0.05). In space exploration experiment, compared with the control group, manganese poisoning mice significantly reduced the number of crossing the platform, and the difference was statistically significant(P <0.05); and compared with the group of manganese poisoning, manganese and vitamin E group and manganese and probucol group mice significantly increased the number of crossing the platform, and the difference was statistically significant(P <0.05); manganese and vitamin E group and manganese and probucol group crossing the platform was no significant difference in the number of times, the difference was not statistically significant(P> 0.05); the number of crossing platform of control group and vitamin E group, no significant difference in the number of mice, the difference was not statistically significant(P> 0.05). 2. Immunohistochemical expression ofcytochrome C: Manganese poisoning group compared with the other four groups, the expression of cytochrome C on hippocampal CA1 and DG regions of manganese poisoning group was significantly increased, and the difference was statistically significant(P <0.05); between the control group and the vitamin E group on hippocampal CA1 and DG zone, the cytochrome C expression was not statistically significant(P> 0.05); while between manganese and vitamin E group and manganese and probucol group showed no significant difference(P> 0.05). 3. Immunohistochemical expression of caspase-3: Manganese poisoning group compared with the other four groups, the caspase-3 positive cells was significantly increased on hippocampal CA1 and DG regions of manganese poisoning group, and the difference was statistically significant(P <0.05); and between the control group and the vitamin E group show that the difference was not statistically significant(P> 0.05); while between the manganese and vitamin E group and the manganese and probucol group showed no significant difference(P> 0.05). 4. Immunohistochemical expression of nestin: Manganese poisoning group compared with the other four groups, the number of positive cells of nestin in the DG zone of manganese poisoning group were significantly reduced, the difference was statistically significant(P <0.05); and between the control group and the vitamin E group show that the difference was not statistically significant(P> 0.05).Conclusion: 1. Vitamin E and probucol may improve the ability of learning and memory on manganese poisoning mice; 2. Manganese poisoning can promote the release of cytochrome C in the brain of mice, the expression of caspase-3 activation, thereby reducing the ability of learning and memory in mice; 3. Vitamin E and probucol can reduce the release of cytochrome C and reduces neuronal apoptosis on manganese poisoning mice; 4. Manganese poisoning can decrease neural stem cells in the DG, vitamin E can increase the number of neural stem cells in manganese poisoning mouse, but probucol has no effect on the neural stem cells of manganese poisoning mouse; 5. Vitamin E has certain protective effect for the manganese poisoning brain damage repair by increasing the number of neural stem cells in the DG of manganese poisoning mouse.