Construction Of Laminin/Fucoidan Similar Basement Membrane Microenvironment And Microfluidic Application

Vascular endothelial layer has a natural anticoagulant effect and rapid endothelialization function.The basement membrane located between endothelial layer and smooth muscel layer can balance the growth behavior of these two kinds cell.Mimic construction basement membrane structure on vascular implants surface can effectively reduce the complications after implantation.Currently, the using of natural biological macromolecules,wich having cell binding sites and three-dimensional structure, to build basement membrane environment is an promising way for material surface biochemical modification and regenerative medicine.Therefore, we use polysaccharide molecules and proteins to bionic construct vascular basement membrane environment, and apply this film into the microfluidic chip to study the interaction between the cells and the constructed films.The main contents are as follows:(1) Layer-by-layer (LBL) assembly of fucoidan sulfate (F) and laminin (Ln) is fabricated on slide surface via APTE silanized by electrostatic attraction.The biocompatibility of self-assembly films is further evaluated. Acid orange experiment、XPS results show rich -NH2 surface is acquired after APTE silanized.Water contact angle of the film show a hydrophilic property, Surface fucoidan content analysis and QCM-D results show that the assembly quantity increasing with the amount of Ln and F. Compared with the control group (glass surface),with the LBL film assembled layers increasing,the properties of anticoagulation and promoting the endothelial progenitor cell growth ability increase.(2) Blending films withfucoidanand laminin are fixed to glass surface after APTE silanized.The stability and biofunctionality of Ln/F film formed under pH7 conditions was investigated, including blood compatibility,endothelia cells (EC) compatibility, anti-hyperplasia properties.and inflammation compatibility XPS results show -NH2, SO42-groups successfully fixed to the surface,and the results of fluorescence microscopy and scanning electron microscopy images show that the slide surface film morphology.Fucoidan content analysis and QCM-D are used to quantity the of Ln/F assembly. The results show that the sample of G@A@200Ln/100F could significantly inhibits platelet adhesion and activation, and promoting endothelial cell growth, inhibiting smooth muscle cell growth and anti-inflammatory response properties in cell culture experiment.(3) By compared with the stability of LBL and blending methodtBlending F and Ln method is applied for microfluidic channel, In the endothelial cell culture experiment, The M+Ln+F microenvironment demonstrat good ability to promote endothelial growth than control group(matrigel environment). Similarly, the SMC culture experiment show, M+Ln +F microenvironment have a better ability to inhibit smooth muscle cell growth than M micro environmental.Through the EC/SMC co-culture experiment, M+Ln+F modified channel show a better function to promote endothelial cell growth and inhibition smooth muscle cell growth. In summary, in this paper Ln/F multifunctional films are successfully constructed on to glass surface and applied to microfluidic chips to evaluate vascular cells growth.It has an important significance to promote microfluidic microarray technology application in the field of implant materials research.