Relationship Of Polymorphism Of Interleuk In-10-819T/C Gene And Interferon-Γ+874T/a Gene With Prognosis Of Hepatitis B Virus In Fection In Guangxi Population

OBJECTIVE To investigate the relationship between interleukin 10 and interferon-y gene polymorphism with prognosis of hepatitis B viru s infection in Guangxi population. This study examined the association of the interleukin-10 gene 819T/C locus and interferon-y+874T/A gene poly morphism with hepatitis B virus infection with different clinical types.METHODS The single nucleotide polymorphisms of IL-10-819T/ C and IFN-γ+847T/A were detected in 254 cases of chronic HBV infectio n, including 48 chronic HBV carriers,148 cases of chronic hepatitis B, 58 cases of HBV-related liver cirrhosis, and 56 healthy controls by pol ymerase chain reaction-restriction fragment length polymorphism (PCR-RF LP) and polymerase chain reaction-sequence-specific primers (PCR-SSP). The distribution frequencies of genotype and allele were observed in patie nts with different clinical types of chronic HBV infection as well as healt hy controls.RESULTS liver cirrhosis, chronic hepatitis B, carrier groups Ⅰ L-I0-819 sites TT/TC/CC genotype frequencies with the control group, th e difference was not statistically significant (P>0.05). And cirrhosis group IL-I0-819T/C locus T allele frequency differences between the control gr oup was statistically significant (P<0.05). Cirrhosis group T allele (73.3%) was significantly greater than in the control group (57.1%). IL-I0-819T/C locus allele frequency of chronic hepatitis B differences between the cont rol group was statistically significant (P<0.05). T allele of chronic hepatiti s B (67.7%) was higher (57.1%). IL-I0-819T/C locus genotype and allele frequency distribution in the cirrhosis group HBV-DNA<1×103 copies/mL n egative group and HBV-DNA≥1×103 copies/mL positive group the differenc e between the two groups, there was no statistical significance (χ2=0.22 8, P=1.000; χ2=0.790, P=0.778). IL-I0-819T/C locus genotype and all ele frequencies of HBV-DNA replication level grouping in chronic hepatiti s B also no significant difference at comparison (χ2=2.228, P=0.328;χ2 =2.988, P=0.084). Statistically significant IFN-γ gene+874T/A locus gen otype and allele frequency differences in liver cirrhosis group and the con trol group in. IFN-γ+874T/A locus genotype and allele frequency differenc e was statistically significant (P<0.05) in chronic hepatitis B group and c ontrol group. IFN-γ+874AA genotype frequency in liver cirrhosis group (8 4%), chronic hepatitis B (79%) was significantly higher than the proporti on of the healthy control group (60.1%). IFN-γ+874T/A locus genotype an d allele frequency distribution in the cirrhosis group HBV-DNA<1×103 copi es/mL negative group and HBV-DNA≥1×103 copies/mL positive group in th e two groups was not statistically significant (χ2=1.777, P=0.516;χ2= 0.953, P=0.329). Chronic hepatitis B HBV-DNA replication relatively lo w levels of IFN-γ+874T/A locus genotype and allele frequency distribution of the difference are not statistically significant (χ2=0.806, P=0.678;χ 2=1.000, P=0.317)CONCLUSIONS IL-I0-819T/C site SNP may be associated with different clinical types of the body after infection with HBV. IL-I0-819T/ C site T allele gene may influence the chronic hepatitis and cirrhosis of t he outcome of HBV infection. IFN-y+874T/A gene site SNP may affect d ifferent outcome of HBV infection; IFN-y gene promoter+874AA genotype and A allele gene may be affects the process of a low clinical outcome of HBV infection.