The Relevance Of Genotypes Of Hepatitis B Virus(HBV) With Th1/Th2 Immune Response And Different Clinical Symptoms Of Hepatitis B Patients In Hefei District

Objective To study the correlation of the distribution of hepatitis B virus(HBV) genotype using different types, serum levels of IFN-γ, IL-6, IL-10 and clinical symptoms of HBV patients in Hefei. Methods 30 sera of healthy persons and 192 sera of patients with HBV infection, including 38 asymptomatic carriers, 109 cases of chronic hepatitis, 25 cases of cirrhosis and 20 cases of hepatocellular carcinoma. HBV DNA were extracted from infected patient, and the HBV genotypes and subtypes were identified according to the fragments size of the products after nested PCR and digestion. The relevance of HBV genotypes and clinical symptoms was analyzed. The HBeAg was detected of by ELISA, and HBV DNA by Fluorescence quantitative PCR in sera of HBV patients. The correlation between HBV genotypes and HBeAg and HBV DNA concentration was analyzed. The levels of IFN-γ, IL-6, IL-10 with ELISA were obtained from 30 serum samples of healthy individuals and 192 copies of HBV infection. After that, it’s studied of the correlation about IFN-γ, IL-6, IL-10, HBV genotypes and clinical symptoms in HBV infection. Results Among 192 HBV-infected patients, the proportions of genotype B, C, and B/C were 42.7%(82/192), 51.6 %(99/192), and 5.7 %(11/192), respectively.In genotype B,57(69.5 %) were subtype Ba, 8(9.8 %) subtype Bj, and the remaining 17(20.7 %) were neither Ba nor Bj. In genotype C, 8(8.1 %) were subtype C1, 56(56.6 %) were subtype C2, and the other 35(35.4 %) were neither C1 nor C2. In genotype B/C,6(54.5 %) were Ba/C2, 2(18.2 %) Bj/C2, 2(18.2 %) Ba/ neither C1 nor C2, and 1(9.1 %) neither Ba nor Bj/C2. It was found that the distribution of HBV genotype and subtype exhibited significant differences in the various clinical types of HBV infection tested,and showed that genotype C was predominant among patients with liver cirrhosis(72.0 %) and hepatocellular carcinoma(85.0 %), while genotype B was predominant in asymptomatic carriers(81.6 %). In addition,in different gender patients, there is no difference of HBV genotype distribution,but genotype distribution showed significant difference between HBeAg positive and negative patients. Comparison of the HBV DNA concentration of the different genotypes, it was statistically significantly higher in genotype C than genotype B. The serum IFN-γ, IL-6, IL-10 levels of healthy human were significantly higher than the HBV patients by ELISA. IFN-γ and IL-10 of patients with chronic hepatitis B, liver cancer patients and in patients with liver cirrhosis showed a rising trend, while the IL-6 was no significant differences in serum of HBV patients. It was found that the levels of IFN-γ higher in genotype B than genotype C significantly, while no significant difference of the IL-6 and IL-10 levels was observed between each genotype. Conclusion It’s dominant of genotype B and C of HBV infection in Hefei City, and genotype C may correlate with liver cirrhosis and hepatocellular carcinoma. More patients infected genotypes B were HBeAg negative, while the genotype C is more in HBeAg positive patients. IFN- γ and IL-10 increased gradually in the patients with chronic hepatitis B, liver cancer and liver cirrhosis. The levels of IFN-γ in serum of the genotype B patients were higher than that of the genotype C, however IL-6 and IL-10 were not significantly different.