The Effect Of Matrine In The Adriamycin-induced Nephropathy Rats And The Expression Of Podocin And α-actinin-4

Objective:1.To observe the protection of matrine in the adriamycin-induced nephropathy rats.2.To study the expression of podocin and α-actinin-4 in adriamycin-induced nephropathy rats.Methods:Divide 50 SPF rats randomized in 5 group. Feed rats adaptively for 1 week after removed their left kidney. The rats nephropathy was induced by two injecting adriamycin at 2-week intervals(1st dose:5mg/kg,2nd dose:2.5mg/kg). Groups:control group(A), sham operation group(B), model group(C), ADR+Matrine 25mg/kg·d group(D), ADR+Matrine 50mg/kg · d group(E). At 2nd,4th,6th week after the establishing of models detect the 24 hours protein of rats urine. Calculate kidney of rats for pathological examination(light microscopic and Electron microscopy), study index of glomeruli sclerosis and detect the expression of podocin and α-actinin-4 at 6th week.Results:1.There was no significant difference between group A and group B in pathological examination and 24 hours protein of rats urine.2. The results of light microscopic examination shows that glomeruli suffer from wide ranges of glomeruli sclerosis under light microscopic in HE,PAS, Masson stain in group C. The capillary lumens surround glomeruli expanded, and the fibers proliferated in renal tubulointerstitial. Under observation of electron microscopy we can confirm the fusion of podocyte foot processes arid the irregular thickened basal membrane. The epithelial cell of renal tubular lost microvilli, with swelling endothelial cells. The mesangial cells also proliferated, extracellular matrix increased,and there are huge amount proliferation of fibers.Compare control group to model group, the 24 hours protein of rats urine of model group get higher than control group, and continue increased over time. By the observation of pathological biopsy, we found that there are big difference between the index of glomeruli sclerosis in group C and group A(P<0.05).The result of Western-Blot shows the expression of podocin and α-actinin-4 get lower in group C than group A, B(P<0.05)3.After the matrine treatment,the results of light microscopic examination in HE ,PAS, Masson stain shows less glomeruli sclerosis in group D and E although the thickened basal membrane and proliferation of fibers still exist. The observation of electron microscopy shows lighter fusion of podocyte foot processes, witch slightly injured than group C. At 2th week, the 24 hours protein of rats urine in group D,E get higher than group C (P>0.05), and decreased begin 4th week. At 6th week, the 24 hours protein of rats urine in group D,E get Lower than group C (P<0.05), and still high than group A (P<0.05).The pathological biopsy confirm that the index of glomeruli sclerosis have lower levels in group D and group E compare with group C(P<0.05).The result of Western-Blot shows the expression of podocin and α-actinin-4 get higher than group C.Conclusion:1. The marine can protect kidney in the adriamycin-induced nephropathy rats.2.the matrine can promote the expression of podocin and α-actinin-4 in adriamycin-induced nephropathy rats, so that get the glomeruli slightly injured and delay glomeruli sclerosis.