| Background micro RNAs are a highly conserved class of small endogenous nonprotein-coding RNAs that present in eukaryotes widely. They can regulate translational repression or degradation by targeting the coding sequences of specific m RNAs. It has also been suggested that overexpression or down-regulation of mi RNAs may induce disregulation of specific m RNAs. Mi RNA involved in many physiological and pathological processes, and plays a key role in the regulation of normal immune function and inflammation, including innate immunity, the adaptive immune. Ankylosing spondylitis(AS) is a chronic inflammatory rheumatic disease characterized by inflammation of spinal and sacroiliac joints, which initially causes bone and joint erosion and subsequent ankylosis. The etiology and pathogenesis of AS is still unclear, which may be related to genetic, immune responses, genetic interaction with the environment and so on. Cytokines play a signaling role in the growth, differentiation and immune regulation of the immune cells. Previous study by our research team discovered the abnormal expression of cytokines in the AS, such as IL-6, IL-12 and IL-17. In order to provide evidence for etiology and treatment of ankylosing spondylitis,we measured the expression of mi RNAs in AS patients,and analysis the relationship between immunocytokines relevant.Objective The aim of our study was to explore the correlation between mi RNA and AS by examining the expression of mi R-155, mi R-16, mi R-31, mi R-181 a in peripheral blood mononuclear cells, and analyzing the relationship between immunocytokines,disease activity and laboratory parameters.Methods The expression of mi R-155, mi R-16, mi R-31, mi R-181 a in peripheral blood mononuclear cells from 40 patients with AS, 40 normal subjects was analyzed by quantitative reverse transcription-polymerase chain reaction(RT-PCR). Patients with AS were from the Rheumatology Department of Anhui, Provincial Hospital and First Affiliated Hospital, Anhui Medical University. AS patients was divided into disease activity group and stable condition group according to the disease activity. 40 age-and sex-matched healthy controls(HC) was selected during the same period from the blood center in He Fei. Sandwich enzyme-linked immunosorbnent assay(ELISA) was used to measure the levels of IL-6, IL-12, IL-17, IL-10, TNF-a in serum in AS patients, and measure the levels of IL-10, TNF-a in serum in healthy controls. The relationship between each mi RNAs expression and immunocytokines, disease activity and clinical indicators was analyzed.Results The study found that mi R-31 expression in peripheral blood mononuclear cells of AS patients was significantly higher than in healthy control group(Z=-4.007, p<0.001), but no significant difference was observed in mi R-155, mi R-16, mir-181 a between AS and healthy control group(Z=-1.095, P=0.274; Z=-1.555, P=0.120; Z=-1.720, P=0.086). There are no significant in mi R-155, mi R-16, mi R-31, mi R-181 a between disease activity group and stable condition group(Z=-0.525, P=0.607; Z=-0.818, P=0.421; Z=-0.812, P=0.434; Z=-0.66, P=0.517). Meanwhile, the expression of mi R-155, mi R-16, mi R-181 a, mi R-31 was no positively correlated with IL-6, IL-12, IL-17, IL-10, TNF-a(P> 0.05). And the same result to the score of BASDAI, ESR, CRP(P> 0.05). IL-6, IL-12 is associated with BASDAI(P<0.05.)Conclusion The expression of mi R-31 was up-regulated in peripheral blood mononuclear cells of patient with AS. It might play an important role in the pathogenesis of AS. |
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