| Objective:The traditional antineoplastic therapy target is always not accurate,meanwhile bring many adverse reactions to human. In order to avoid adverse reactions,we hope the drug release only after reach target site. Our subject is based on mesoporous silican(MSN),gold nanoparticles(Au NPs) and folate acid(FA) are absorbed on the surface of MSN, we use doxorubicin hydrochloride(DOX) as drug model,loading into mesoporous,so that the prepared nanomaterial posses thermo-therapy,chemotherapy.Method: The first step is direct synthesis of amino mesoporous silicon nanomaterials, after the mesoporous silica nanoparticles treatment with diluted acetic acid, the amino group protonated, weakening the interaction between the hydrogen bond,so that mesoporous silica will be monodisperse nanoparticles, while improving the electrostatic adsorption of gold nanoparticles. Use EDC and NHS-activated carboxyl group of folic acid, folic acid and mesoporous silicon surface carboxyl amino group acylation reaction, the folate-linked mesoporous silicon surface. Using UV-spectrophotometer to detect folic acid content on MSN. The MSN@Au-FA, by physical principles contained negative doxorubicin, using fluorescence spectrophotometer to detect drug loading rate and encapsulation efficiency. At 37℃, PH=7.4 PBS solution, investigate in vitro drug release conditions; hemolysis test of the nanomaterials, study the biological safety of blood; the MSN @ Au-FA / DOX nanoparticles incubate with mouse melanoma cells(B16),, human ovarian cancer cells(Hela),human hepatoma cells(Hep-G2) 12 h,the result showed that our nanomaterials are safety.The cell inhibition experiment showed that MSN、MSN@Au、MSN@Au-FA generate hyperthermia to kill the cancer cell.Conclusion: The resultsof folate-modified drug nanoparticles in vitro experiments showed folic acid modified drug nanoparticles with tumor cell binding rate;nanoparticles containing gold particles significantly generate hyperthermia. |
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