| Objective: Taking Bu Gu Zhi-Rou Dou Kou Herb pair as the research object, on the basis of literature research, the application of modern science and technology method of Bu Gu Zhi-Rou Dou Kou Herb pair fingerprint changes and provided medication position before and after compatibility of ethanol as a qualitative and quantitative analysis of components of research, aims to change clearly Bu Gu Zhi-Rou Dou Kou Herb pair ethanol extract parts of compatibility of medicine before and after the main chromatographic peaks, reveal the Bu Gu Zhi-Rou Dou Kou Herb pair on compatibility mechanism maybe from the chemical compositionangle; clear ethanol extract of Bu Gu Zhi-Rou Dou Kou Herb pair parts contained in the chemical composition of information, for the establishment of Bu Gu Zhi-Rou Dou Kou Herb pair on the quality control standard for Reference.Provides the reference of the experiment is containing the drug to the modern preparation composition study.Method: Based on summarizing Bu Gu Zhi-Rou Dou Kou Herb pair on the research status at home and abroad, the main research contents including: Bu Gu Zhi-Rou Dou Kou Herb pair extraction process research on ethanol; Bu Gu Zhi-Rou Dou Kou Herb pair mention studies on changes of UPLC fingerprint of compatibility of parts before and after ethanol; Bu Gu Zhi-Rou Dou Kou Herb pair on ethanol extract sites in the chemical composition of UPLC/Q-TOF-MS and UPLC-PDA quantitative analysis of qualitative research.1. Study on extraction process of Bu Gu Zhi-Rou Dou Kou Herb pairThe experiment used UPLC fingerprinting combined with orthogonal test for optimization of Bu Gu Zhi-Rou Dou Kou Herb pair the optimum ethanol extraction process. Ethanol concentration, ethanol dosage, extraction time, extraction times of4 factors, using L9(34) orthogonal test, with UPLC fingerprinting of total peak area,psoralen, angelicin, dehydrodiisoeugenol and yield of dry extract as indexes,using comprehensive score for data analysis method, the selection of optimal alcohol extraction process.2.Study on the changes of Bu Gu Zhi-Rou Dou Kou Herb pair provided UPLC fingerprint of parts before and after compatibility of ethanolThis experiment adopts the UPLC-PDA method of fructus BuGu Zhi single medicine,Rou Dou Kou single medicine,Bu Gu Zhi-Rou Dou Kou Herb pair for alcohol extract features offingerprint and psoralen myristate drugs on 7 known components of quantitative research, the influence of compatibility on the drug on the chemical composition of the. The experimental set up the 10 batch of single herbs of Bu Gu Zhi and Rou Dou Kou, 10 batches of Bu Gu Zhi-Rou Dou Kou Herb pair for alcohol in the same conditions of extract of UPLC fingerprinting, the establishment of the above 3groups of fingerprint of their respective common mode chart, and calibration of their common peaks of fingerprint, gain control of the self the. Preliminary common peak qualitative analysis and drug content of alcohol extract of psoralen and Angelicin, Bavachin, corylin, Isobavachalcone, Dehydrodiisoeugenol and bakuchiol solution.3.Ethanol extract of Bu Gu Zhi-Rou Dou Kou Herb pair analysis qualitative and quantitative research site UPLC-PDA and UPLC/Q-TOF-MSIn order to fully quickly clarify Bu Gu Zhi-Rou Dou Kou Herb pair chemistry composition and peakposition on alcohol attribution, ultra performance liquid chromatography and tandem quadrupole time of flight mass spectrometer(UPLC/Q-TOF-MS)based on the same experimental conditions of Bu Gu Zhi-Rou Dou Kou Herb pair and mixed reference chromatograminto UPLC/Q-TOF-MS, combined with literature and Mass Lynx workstation Exact Mearch(EMS) software mass spectrometry database retrieval, identification and preliminary identification of unknown compounds of known compounds.Experiment with UPLC-PDA method of Bu Gu Zhi-Rou Dou Kou Herb pair in extract of psoralen and Angelicin, Bavachin, corylin, Isobavachalcone, Dehydrodiisoeugenol and bakuchiol solution.Result:1.Experimental science, effectively optimized extraction process on the optimal alcohol: ethanol concentration 60%, dosage of ethanol 8 times, each time 1extraction h, extraction for 2 times.2.Bu Gu Zhi-Rou Dou Kou Herb pair cues on the results of UPLC fingerprint : after the combination of single Bu Gu Zhi elcohol extract peaks was 27, single Rou Dou Kou elcohol extract ofnutmeg peaks was 10, while the Bu Gu Zhi-Rou Dou Kou Herb pair extracts of elcohol common peak but no 37. 27 peaks from single Bu Gu Zhi in Bu Gu Zhi-Rou Dou Kou Herb pair on total peaks were retained, and the peak area of the 27 peaks of Bu Gu Zhi increase. 10 peaks from Rou Dou Kou single drug,in which the 7 peak of alcohol in Bu Gu Zhi-Rou Dou Kou Herb pair provided nutmeg medicine liquid disappears, the other 3 peak area peak average value were decreased.3.The experiment based on UPLC/Q-TOF-MS high resolution mass spectrometry data and mixed control sample information of Bu Gu Zhi-Rou Dou Kou Herb pair on the chromatographic peaks were identified, summed up the law of cracking Bu Gu Zhi-Rou Dou Kou Herb pair of coumarins, flavonoids, monoterpene phenols and lignans, further identification and improve 7 known compounds information,and preliminary identification of 12 unknown compounds.The experiment according to the UPLC-PDA 10 batches of Bu Gu Zhi-Rou Dou Kou Herb pair mention 7 known compounds in parts of alcohol in quantitative analysis.Conclusion: This experiment was optimized to Bu Gu Zhi-Rou Dou Kou Herb pair extraction process of alcohol, and carries on the extraction process showed that the process route is reasonable, feasible; experiment with UPLC legal weight determination, fingerprint and UPLC/Q-TOF-MS technology for Bu Gu Zhi-Rou Dou Kou Herb Pair on chemical composition were studied, to a more comprehensive information to obtain compounds for the drug, and the experimental method has the advantages of simple, good repeatability etc. |
PDF Full Text Request