Effects Of Blocking MAPK/ERK Signal Pathway On Proliferation And Invasion Of Ovarian Cancer Cells

Object:To investigate the effects of PD98059, an inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase(MAPK/ERK) pathway on the proliferation and invasive abilities of ovarian cancer SKOV3 and OVCAR3 cells, and to explore the possibility of ovarian cancer treatment by blocking the MAPK/ERK signaling pathway. Methods:The SKOV3 and OVCAR3 cells were cultured in vitro, and treated with different concentrations of PD98059 for different times. Then the proliferation activity of SKOV3 and OVCAR3 cells was detected by cell counting kit-8(CCK-8) assay. The expression level of phospho-ERK protein was detected by Western blotting. The invasion ability of SKOV3 and OVCAR3 cells was detected by Transwell invasion experiment. Conclusion:PD98059 inhibited the proliferation of SKOV3 and OVCAR3 cells in a concentration- and time-dependent manner. PD98059 inhibited effectively the phosphorylation of ERK1/2, namely down-regulating the expression of p-ERK1/2 protein in cancer cells, so that the activity of ERK1/2 signal transduction pathway was blocked. Moreover, PD98059 in a certain concentration range could inhibit the invasion of these two ovarian cancer cells.