Researches On The Expression Of HP1γ In Bladder Urothelial Carcinoma And Its Biological Behavior Influence On Bladder Urothelial Carcinoma Cells

Objective 1,To identify the expression of HP1γ in bladder urothelial carcinoma, and analyze the correlation with clinic pathological parameters,as well as its prognostic function.2, To investigate the effect of HPly on proliferation, colony forming ability, and transfer ability of bladder urothelial carcinoma cells,T24 and 5637, providing a new target for diagnosis and treatment of bladder urothelial carcinoma.Methods 1,Test the expression of HP1γ in 30 pairs of bladder urothelial carcinoma and their adjacent normal urothelium by Western Blot.Test the expression of HPly, Ki67 in 62 bladder urothelial carcinomas, then analyze the relationship with clinic pathological parameters,as well as its prognostic function.2, Downrergulate the expression of HPly in T24 and 5637,two bladder urothelial carcinoma cell lines selected to be studied. Test the change of proliferation,colony forming,and transfer by MTT assay,Soft Agar assay and Scratch assay.Finally,test the expression of genes related to glycolysis.Results 1.The expression of HPly in bladder urothelial carcinoma is higher than that in adjacent normal urothelium (19/30).It is related to the clinical stages,including whether muscle invasive and whether lymphatic metastasis or not, it is an independent prognostic factor.2. The proliferation,colony forming,and transfer ability were declined due to the downregulation of HPly, G1 block was observed.The expression of Glutl, HK2, PGK, LDHA were also downregulated.Conclusion HPly highly expresses in bladder urothelial carcinoma, and is likely to be a potential tumor marker for bladder urothelial carcinoma. Downregulation of HPly inhibited the tumor behavior, suggesting that it is an ideal target for cancer molecular targeting therapy.