Kidney Invigorate Soup Regulated OPG/RANKL/RANK Signaling Pathway For Steroid-Induced Avascular Necrosis Of Osteoblasts And Osteoclasts Influence

ObjectiveBy observing Bushenhuoxue decoction on SD rat steroid-induced avascular necrosi s of the OPG/RANKL/RANK signaling pathway to explore Bushenhuoxue soup SD r ats steroid-induced avascular necrosis of the OPG/RANKL/RANK signal pathway mec hanism to explain the treatment of hormone soup Bushenhuoxue avascular necrosis of the mechanism, of Bushen Decoction on steroid-induced avascular necrosis of osteobla sts and osteoclasts impact.Methods1.64 SD rats, grouping with random number table, divided into 14 groups, maki ng the module 50, making the module using LPS combined hormone modeling metho d of modeling. Normal group were injected with the same dose of the same frequenc y saline. After six weeks, the control group and model group were randomly selected 2,10% chloral hydrate intraperitoneal injection of anesthesia after removing fresh S D rat femoral tissues histological testing, validation of modeling results.2. After successful modeling by human and rat body surface area dosage conversi on, calculate the required dose for each rat, Kidney Invigorate Soup high, medium an d low-dose group were given crude drug concentrations were 2.5g/kg,5g/kg, lOg/kg of Bushenhuoxue soup orally, once a day, morning and evening; normal and model groups in the first 7 to 10 weeks, with saline 5g/kg/d of gavage, once a day, morn ing and evening. Containing crude drug concentration of 1.0g/ml Bushenhuoxue soup preparations computing, low-dose group was given a dose of 2.5ml/kg, medium-dose group was given a dose of 5ml/kg, high-dose group was given a dose of 10ml/kg.3. The five groups, the celiac artery blood, blood serum, serum ALP content rats, femoral heads light microscope femoral bone tissue changes, the use of protein immu noblotting technique to detect SD rat femoral the expression of RANKL, TRAF-6, us ing immunohistochemistry femoral OPG expression.Results1. Small doses of LPS combined hormone modeling method necrosis rate, the mo del shows necrotic cells large cloud sheet, the tide line is not continuous or tear; trab ecular bone fracture, necrosis disappeared, empty lacunae was 32.48±2.43, animals 1 ow mortality rate (3.125%), modeling cycle is short, treatment methods and clinical pa tient modeling of closer.2. Serum LAP detection:high-dose Bushenhuoxue soup can effectively improve th e body content of rat serum LAP, the high doses of Bushenhuoxue Infusion SD rat f emoral head cartilage layer of connective tissue newborn osteoid trabecular bone subst antially continuous, necrosis was significantly lower than in the low-dose group and model group, has good osteogenesis.3. Femoral head OPG expression of immunohistochemical staining detection:Kidn ey Invigorate Soup decoction of the rats lavage OPG expression within the femoral he ad higher than the model group, difference have statistical significance; High dose kid ney invigorate decoction lavage group of OPG immunohistochemical staining in the av erage absorbance and positive expression rate is higher than obviously, low dose grou p, the difference is statistically significant. Show that kidney osteogenesis effects of hu oxue soup, high doses of kidney invigorate curative effect is best.4. Femoral head RANKL, TRAF-6 immune protein imprinting table detection:k idney huoxue soup decoction of the rats in the femoral head by gastric RANKL, TR AF-6 expression is lower than the model group, difference have statistical significan ce; High dose kidney huoxue decoction lavage group of RANKL, TRAF-6 positive expression under middle and low dose group, the difference is statistically significant. Show that kidney-huoxue soup with femoral head broken bone factor RANKL, TRAF-6 expression effect, high doses of kidney huoxue soup curative effect is best.Conclusion1. The low-dose LPS combined hormone modeling method necrosis rate, mortality rate is low, the modeling cycle is short, treatment methods and clinical patient model ing of closer is an effective method of modeling.2. High-dose Kidney Invigorate Soup can effectively improve the serum in vivo r at LAP-tech, high-dose Bushenhuoxue Infusion of SD rats was significantly lower than in the femoral head necrosis low-dose group and model group, with better osteogenic effect.3. Kidney Invigorate Soup can enhance the expression of OPG, with osteogenesi s, the efficacy of high-dose Bushenhuoxue best soup; Bushenhuoxue soup femoral inhi bit osteoclast factor RANKL, TRAF-6 Expression, high doses of Bushenhuoxue soup b est effect.