To Study The Expression And Correlation Of VWF And TSP-1 In Peripheral And Placental Tissue Of Patients With Preeclampsia

Preeclampsia(PE) is a unique disease in pregnancy, which has serious impact on maternal and children’s health. So far, its pathogenesis is not entirely clear. At present, domestic and foreign scholars believe that the incidence of preeclampsia is closely related to vascular endothelial cell damage and inhibition of angiogenesis. The basic pathology of preeclampsia is vasoconstriction of the whole small blood vessels, causing dysfunction of multi-systems and multi-organs, including the placenta microcirculation. "Vascular recasting disorders" is considered the key to preeclampsia. Von Willebrand factor(v WF), a multifunctional glycoprotein which synthesised and secretioned by vascular endothelial cells, is the important initiation factor of extrinsic coagulation system and thrombosis. v WF can bind to the platelet membrane glycoprotein protein, activated platelet, induce adhesion movement and the formation of platelet thrombi. Thus inhibites the endothelial cells’ s function of repairment and angiogenesis, leading to high blood pressure. Thrombospondin-1(TSP-1), secreted by endothelial cells, is an important inhibitor of Endogenous angiogenesis. As an important factor of inhibiting angiogenesis, TSP-1 can combine to its receptor CD36, resulting in apoptosis of blood vessels endothelial cell and inhibition of angiogenesis.Objective:By studying v WF and TSP-1’s expression in peripheral blood and placenta in preeclampsia and the relevance between them, to explore their influences in onsetion and progression of preeclampsia. So as to provide a theoretical basis on preeclampsia’s early diagnosis, prevention and treatment.Methods:We randomly collect 60 cases of pregnant women with preeclampsia as case group, deliveryed in the Maternity and Child Care Hospital of Shijiazhuang between December 2013- March 2014. Among which 30 cases are in mild preeclampsia group(m PE) and 30 cases are in severe preeclampsia group(s PE). Choose 30 cases of normal pregnancy women in same period as the control group. Enzyme-linked immunosorbent assay( ELISA)method was used to detect the expression of v WF and TSP-1 in peripheral. Using immunohistochemistry techonology to detect v WF and TSP-1’s expression in placenta. In blank control group, the first antibody was replaced by PBS. Using statistical software SPSS13.0 to statistically analysis the datas obtained by experiment. Measurement datas are presented as mean ± standard deviation( x ± s). Count datas are presented by percentage. Compared the mean date among mult-samples, we should test the homogeneity of variance. Yes, we use One-way analysis of variance. No, we use t’ test. Two samples we use t test. Compare the sample rate using the χ2 test. Coefficient of product-moment correlation of pearson is applied to analyze the relationship of two variables, with a test level α <0.05.Result:1 Comparison of v WF’s concentration in plasmaThe average concentration of v WF in plasma is 1.55 ± 0.19 ng / ml in control group, 1.94 ± 0.26 ng / ml in m PE group, 2.15 ± 0.32 ng / ml in s PE group. v WF’s concentration in m PE group and s PE group is higher than in control group, the differences were statistically significant(P<0.05), and with exacerbations showed an increasing trend.Comparison of TSP-1’s concentrations in serumThe average concentration of TSP-1 in serum is 1.31±0.12 ng / ml in control group, 1.52±0.15 ng / ml in m PE group, 1.60±0.18 ng / ml in s PE group. TSP-1’s concentration in m PE group and s PE group is higher than in control group, the differences were statistically significant(P<0.05). TSP-1’s concentration in s PE group is slightly higher than in m PE group, the difference was not statistically significant(P>0.05).2 There is no significant correlation of v WF’s expression in serum and TSP-1’s expression in plasma in control group(r = 0.278, P>0.05).The expression of v WF in serum and TSP-1 in plasma are positively correlated in m PE group(r = 0.641, P<0.05).The expression of v WF in serum and TSP-1 in plasma are positively correlated in s PE group(r = 0.972, P<0.05).3 Immunohistochemical staining: v WF and TSP-1 are both expressed in trophoblast and interstitial of placenta tissue in control group, m PE group, s PE group. Mainly location in the cell membrane and cytoplasm, a small amount expressed in villous stroma. As follows:v WF: ① Compared with normal control group, v WF’s expression in m PE group and s PE group were significantly increased, the difference was statistically significant(P<0.05). ② Compared with m PE group, the expression of v WF in s PE group was significantly increased, the difference was statistically significant(P<0.05).TSP-1:① Compared with normal control group, TSP-1’s expression in m PE group and s PE group were significantly increased, the difference was statistically significant(P<0.05). ② Compared with m PE group, the expression of TSP-1 in s PE group was slightly increased, the difference was no statistically significant(P>0.05).Conclusion:1 v WF and TSP-1’s expression in peripheral blood and placenta in preeclampsia group were significantly higher than in the control group, suggesting that v WF, TSP-1 are related to the occurrence and development of preeclampsia. And vascular endothelial cells damagement, angiogenesis inhibition plays an important role in the development of preeclampsia.2 In patient of preeclampsia, the more serious injury of endothelial cells is, the higher the level of v WF’s expression, suspect that v WF can reflect the severity of preeclampsia.3 TSP-1’s expression in preeclampsia is no significant change with the severity of angiogenesis inhibition. Suspect that TSP-1 expression is related to the incidence of preeclampsia, but the relationship with the severity of the disease is not close.