| Objective: Gastric cancer, one of the common malignancies in clinic, has been a serious threat to the health and lives of residents in our coutry. The incidence of gastric cancer ranks second among all kinds of tumors in men, and the third in women.According to the prediction of epidemiology,the morbidity and mortality of gastric cancer in China will keep the sustained growth trend in the following two decades.The occurrence,development and prognosis of gastric cancer owns to the multiple steps,links,and gene participation. Due to the lack of the effective census methods, we mainly treated advanced gastric cancer patients in clinic.Early diagnosis and effective treatment of gastric cancer have become a problem which needs to be solved.Now,effective chemotherapy is one of the important methods to prolong survival period of patients with gastric cancer, and also a research hotspot in recent years.Gerdes and other scholars created the Ki67 antibody in 1983. Ki67 is a large protein molecule existing in the cell nucleus, with a unique molecular structure that has no apparent homology with any other known proteins.It is involved with cell proliferation activity.LRP is a new multi-drug resistance related proteins,which is found by Scheper and others from a multiple drug-resistant strains cells screened from human small cell lung cancer.The multiple drug resistant cells perform a drop of energydependence of drug accumulation ability.LRP exists in the cytoplasm, causing MDR resistance to platinum drug, alkylating agent-drug and so on.The relationship of LRP gastric cancer prognosis is rarely reported.There are few literatures in which analyzes the correlation of LRP and Ki67,and their relationship with gastric cancer prognosis. [1]This experiment exploved the relationship of their expression and clinical pathologic factors,such as gender, age, infiltrating depth, tumor stage, lymph node metastasis and so on,and also analyzed their clinical significance through the SP immunohistochemical staining of gastric cancer tissues, pericarcinoma tissues,and normal gastric mucasa tissues.The experiment may provide a theory basis for the prognosis of gastric cancer,and for judging and guiding the possibility of chemotherapy.Methods: Using the SP immunohistochemical staining to detect the expression of Ki67 and LRP in the gastric cancer tumor tissues of 40 patients, the corresponding tissue adjacent to carcinoma(cancer 5 cm), 20 cases of normal gastric mucosa tissues,and is combined with the patient’s gender, age, infiltration depth, degree of differentiation, TNM staging for statistics analysis.The test analysis of data is the expression of Ki67 and LRP in gastric cancer correlation through statistical analysis.Difference was statistically significant( P<0.05).Results: 1 The expression level of Ki67 in stomach, tissue adjacent to carcinoma and normal tissue was 72.5%(29/40), 45%(18/40), 10%(2/20), and the difference among three groups was statistically significant(P < 0.05). 2 The expression level of LRP protein in gastric cancer tissue,the tissue adjacent to carcinoma and normal tissues was 75%(30/40), 52.5%(21/40), and 25%(5/20), and the difference among three groups was statistically significant(P<0.05). 3 The relationship of Ki67 and LRP expression in gastric cancer tissue and clinical pathological factors. 3.1 The expression level of Ki67 in poorly differentiated gastric cancer tissue was 85.19%(23/27),in high differentiated gastric cancer tissue was 46.15%(6/13)(P<0.05). The expression level of Ki67 in phase I + II was 50.00%(6/12), in phase III + IV was 82.14%(23/28)(P<0.05).The expression level of Ki67 in the presence of lymph node metastasis was 82.14%(23/28), the expression level without lymph node metastasis was 50.00%(6/12)(P<0.05). The expression of Ki67 with patient’s age, gender is not statistically significant.(P>0.05). 3.2 Expression level of LRP protein in high differentiated gastric cancer tissue was 61.54%(8/13), the expression level of low differentiated gastric cancer tissue was 81.48%(22/27), but P>0.05.The expression of LRP with the patient’s age, gender, differentiation degree, infiltration depth, correlated with tumor stage, lymph node metastasis is not statistically significant(P>0.05). 4 Using the chi-square test correlation analysis, the results show that Ki67 and LRP protein expression was uncorrelated(P>0.05).Conclusions:1 Ki67 was highly expressed in gastric cancer, and was positively correlated with the differentiation degree, infiltration depth, TNM staging, lymph node metastasis, but was not correlated with the patient’s age, gender. Ki67 may promote normal gastric tissue to gastric cancer, and is related with the progress of gastric cancer.2 LRP was highly expressed in gastric cancer, but was unrelated with differentiation degree, infiltration depth, TNM staging, lymph node metastasis and the patient’s age, gender. LRP expression in gastric cancer shows that the LRP plays an important role in the primary gastric cancer drug resistance.3 The expression of Ki67 and LRP in gastric cancer was unrelated, but both are countable for the proliferation of tumor cells, development degree and the development of primary resistance comprehensive evaluation of tumor,which can indirect assessment of tumor prognosis, and provide guidelines for chemotherapy drugs. |
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