| Objectives ①To establish the model of allergic rhinitis (AR) of Wistar rats.② To observe the expression levels of CCL11, AQP-1 and EOS in the nasal mucosa of AR rats, which is challenged by ovalbumin(OVA), and normal rats.③ To investigate the effect and the mechanism of 18β-glycyrrhetinic acid (GA) on the expression level of CCL11, AQP-1 and EOS in the nasal mucosa of allergic rhinitis rats.Methods ①Setting up the AR model:Male Wistar rats,4-6 weeks old, were randomly divided into normal saline control (NC) group, AR without intervention (AR) group, AR treated with loratadine (LOA) group and AR treated with 18β-glycyrrhetinic acid (18β-GA) group. The rats of AR group, LOA group and 18β-GA group were sensitized by intraperitoneal injection with OVA 0.3 g mixed with Al(OH)330 mg in 1 mL normal saline (NS). It was performed every two days and lasted 14 days. Then the rats of AR group, LOA group and 18β-GA group were challenged by intranasal administration with 2% OVA solution 50 uL into bilateral nasal cavity from the fourteen day. It was performed every day and lasted 7 days. while the rats of NC group were given NS replaced of OVA, The method was same to other groups. To evaluate the AR model with passive cutaneous anaphylaxis (PCA) test and AR symptom scores.② Both LOA and 18β-GA groups were taken LOA and 18β-GA respectively form 21 d, I/day, while AR and NC groups were administrated with NS, The method was same to other groups.③ Observing the results:After the 1st,2nd and 3rd weeks’ intervention, we observed the behavioral changes and recorded AR symptom scores, qRT-PCR was performed to investigate the level of CCL11 mRNA with β-actin as internal contral, immunohistochemical (SP) staining was used to examine the AQP-1 expression level, observing tissue structure of nasal mucosa through applying the methods of HE staining and counting EOS in nasal mucosa of rats through light-microscopy in high power field as the degree of EOS recruitment.④ Data analysis:the experimental data analysised with SPSS 19.0 statistical analysis software.Results ①After administration of intraperitoneal injection, the PCA test result of AR group, LOA group and 18β-GA group was positive, After nasal inhalation, the rats of AR group, LOA group and 18β-GA group appeared tipcal AR symptoms, such as scratching nose, clear nasal discharge flow and sneezing, AR symptom scores were higher than 5 points, suggesting we have established model of AR successfully.② After the 1st,2nd and 3rd weeks, compared with NC, LOA and 18β-GA group, AR group showed typical AR symptoms, AR symptom scores and the expression levels of CCL11, AQP-1 and EOS in the nasal mucosa were improved significantly(P<0.05).③ When compared with AR group, the above statistics in LOA group were down-regulated evidently at different points in time(P<0.05).④ After the 1st week, the above detection results in 18β-GA group were lower than AR group(P<0.05), but still higher than LOA group, After the 2nd week, those statistics approached to LOA and NC group significantly.Conclusions ①The model of AR can be successfully established by OVA stimulated.② The nasal mucosa of normal rats distributed a small amount of CCL11, AQP-1 and EOS. As the models of AR exposed to the allergen, the nasal mucosa of rats were invaded by chronic inflammation, showing the obvious AR symtoms, the levels of CCL11, AQP-1 and EOS in the nasal mucosa were improved significantly.③ 18β-GA administration can down-regulate the expression levels of CCL11, AQP-1 and EOS in the nasal mucosa of AR rats, meanwhile it could ameliorate the symptoms and signs of the AR rats and cast effect on inhibiting the AR progress. |
