Expression Of CXCL4,CXCR3 And The Correlation With Tumor Angiogenesis In Laryngeal Squamous Cell Carcinomas

Objective: The laryngeal carcinoma is a common disease in head and neck cancer. In which, the laryngeal squamous cell carcinoma is the most. The incidence of layngeal carcinoma had a increasing tendency in the past 40 years. Chemokines belong to a certain kind of cytokine family of chemotactic and inductive function secreted by leukocystatin, fibroblasts and endotheliocyte in the stimulus of growth factors, bacterial products, viruses and so on. Chemokine receptors are a category of the cell surface protein with an ability to combine with chemokines. The purpose of this study is to observe the expression levels of CXCL4(CXC chemokine, CXCL4), CXCR3(CKC chemokine receptor, CXCR3) and MVD(Microvessel Density) in the laryngeal cancer tissues and adjacent non-cancerous tissues, and the relation between their expression levels and clinical pathological factors. This test also investigate the correlation between the expression of CXCL4, CXCR3 and MVD in the laryngeal cancer tissues.Method:1 The expressing levels of CXCL4, CXCR3 and MVD with 47 cases of laryngeal cancer tissues and 21 cases of adjacent normal tissues were detected by immunohistochemistry.2 The expression levels of CXCL4, CXCR3 and MVD in laryngeal carcinoma tissues was compared to them in adjacent non-cancerous tissues. The relationship between the expression of CXCL4, CXCR3 and MVD in the laryngeal squamous cell carcinoma tissues and clinical pathological factors was also observed.3 Statistical analysis was carried out with SPSS 13.0 software. In all studies, A P-value <0.05 was considered significant, A P-value <0.01 was set remarkable significant.Results: 1 The expression levels of CXCL4 1.1 Used immunohistochemistry to detect the expression of CXCL4 in 47 cases of laryngeal carcinoma tissues and 22 cases of adjacent non-cancerous tissues. The rate of positive protein expression were 76.7%(30/47) and 27.3%(6/22), respectively, and the former was higher than the latter. The difference was statistically significant(P<0.01). 1.2 There was significant difference between the positive expression of CXCL4 in laryngeal squamous cell carcinoma and clinical stages or lymph node metastasis. However, there was no correlation with tumor pathological grades, smoking, age, gender or clinical classification in the positive rates of CXCL4(P>0.05). 2 The expression levels of CXCR3 2.1 Used immunohistochemistry to detect the expression of CXCR3 in 47 cases of laryngeal carcinoma tissues and 22 cases of adjacent non-cancerous tissues. the rate of positive expression were 63.8%(23/47) and 36.4%(8/22), respectively. The difference was considered remarkable significant(P<0.05). 2.2 There was significant difference between the positive expression of CXCR3 in laryngeal squamous cell carcinoma and lymph node metastasis(P<0.05), but it wasn’t correlated with tumor pathological grades, clinical stages, smoking, age, gender or clinical classification(P>0.05). 3 The expression levels of MVD 3.1 Used immunohistochemistry to detect the enumeration of MVD marked by CD34 in 47 cases of laryngeal carcinoma tissues and 22 cases of adjacent non-cancerous tissues, the quantity were 30.21±6.43 and, respectively. There was remarkably significant difference in statistical analysis(P<0.01). 3.2 There was significant difference between the enumeration of MVD in laryngeal squamous cell carcinoma and clinical stages(P<0.05), lymph node metastasis(P<0.05) and clinical classification(P<0.05). However, there was no correlation with pathological grades, smoking, age or gender(P>0.05). 4 The correlation between the expression of CXCL4, MVD and CXCR3The analysis of protein expression levels of laryngeal cancer tissues showed that the expression of CXCL4 and the enumeration of MVD were positively correlated(r=0.434, P<0.05), besides, the expression of CXCR3 and the number of MVD were also positively correlated(r=0.497, P <0.01).Conclusions:1 The higher expression of CXCL4 and CXCR3 in laryngeal squamous cell carcinoma and the lower expression in adjacent non-cancerous tissues was correlated with tumorigenesis and progression of laryngeal squamous cell carcinoma, which difference was statistically significant.2 The higher expression of CXCL4 in laryngeal cancer was correlated with clinical stages and lymph node metastasis as well as the correlation between the higher expression of CXCR3 and lymph node metastasis, which both suggested that they may be involved in the invation and metastasis of laryngeal squamous cell carcinoma.3 The enumeration of MVD in laryngeal squamous cell carcinoma was higher than adjacent non-cancerous tissues. It was correlated with clinical stages, lymph node metastasis and clinical classification, which indicated that microangiogenesis may play an important role in the occurrence, development and metastasis of laryngeal squamous cell carcinoma.4 The two group of CXCL4 and MVD, CXCR3 and MVD were positively correlated, which demonstrated they maybe collectively participated in the process of microangiogenesis of laryngeal squamous cell carcinoma.