The Effects Of 26RFa On The Differentiation Of Bone Marrow Mesenehymal Stem Cells Into Neuron-like Cells And Osteoblasts In Vitro

Objective:To investigate the effects of 26RFa on the differentiation of bone marrow mesenehymal stem cells(BMSCs) into neuron-like cells and osteoblasts in vitro.Methods:The experiment was divided into 4 groups:group A (26RFa), group B (P-Me-rcaptoethano, BME), group C (26RFa + BME)and group D (control group). The morphological change was observed during inducing. The immunocytochemical technique was used to detect the expressions of NSE, Nestin, and GFAP. The toluidine blue staining were used to assess the neuronal differentiation. In osteogenic differentiation experiment,4 groups was also divided, group A (26RFa), group B(dexamethasone, β-glycerophosphate and ascorbic acid), group C(26RFa and a group), and group D as control group. The morphological change was observed during induction.The alkaline phosphatase(ALP) activity, alkaline phosphatase staining, alizarin red mineral deposition staining were performed to assess the osteogenic capacity.Results:Immunocytochemistry showed that NSE was expressed in A,B and C group, while not express in D group, the positive rate of NSE was significant difference C>B>A>D(P<0.05). Nestin was expressed in A, B and Cgroup, while not expressed in D group, the results were different in different time. No group expressed GFAP. The toluidine blue staining was positive in A, B, C group, negative in D group. The positive cells of alizarin red staining in C>B>A>D(P<0.05). The ALP staining of the cells in A, B and C group was positive, while negative in the control group.The positive of alkaline phosphatase were different in different time.Conclusion:Our study showed that 26RFa has effects on BMSCs that differentiate into neuron-like cells and Osteoblasts in vitro.26RFa can effectively enhance the function of traditional inducer.