The Study Of Silk Fibroin/Collagen/PLLA Nanofiber Scaffolds Crosslinked Modified And Biocompatibility In Vitro

Objective:using the electrostatic spinning technology preparation of silk fibroin/collagen/PLLA nano fiber scaffold materials, research crosslinked modified, the degradation of material properties and biocompatibility in vitro, to explore the potential of its application in tissue engineering.Methods:Using electrostatic spinning technique to prepare various ratios of silk fibroin/collagen/PLLA composite scaffolds, the glutaraldehyde steam crosslinking agent on the nanometer fiber membrane. Fiber morphology was observated by SEM, X-ray diffraction (XRD) and tensile properties test. The degradation of performance is discussed and test the weightlessness and liquid pH. Culture the periodontal ligament stem cells and bone marrow mesenchymal stem cells on the material, through MTT and cell SEM to observe cell proliferation and adhere, detecting biocompatibility in vitro.Results:The best crosslinking time is 48h. Single fiber after crosslinking is swelling. Silk configurations are mainly composed of beta fold. Fiber crosslinked has a lot to improve mechanical properties. And when the silk fibroin/collagen with PLLA is 30:70, the fracture stress is the largest. Silk fibroin/collagen membrane degradation of the slowest, pure PLLA degradation was fastest. But the silk fibroin/collagen to join, make PLLA degradation rate slows. Determined by MTT and SEM showed that silk fibroin/collagen/PLLA nanometer fiber membrane after crosslinking still has a good biocompatibility in vitro, when the SF/COL:PLLA is 30:70, the two kinds of cells on the scaffold adhesion, and spreading most.Conclusion:glutaraldehyde on silk fibroin/collagen/PLLA scaffold materials best crosslinking time is 48 hours, when the SF/COL:PLLA as 30:70, exerts good tensile properties, and the most beneficial to the stem cells...