The Chemical Crosslinking And Modifying Of Silk Fibroin Fiber

The cells adhere well on the fibers whose dimension is as same as their own (mieroscale) or below their own so that the porous three dimension electrospun scaffold material can mimic the structure of the natural extracellular matrix better and have the potential to be an ideal tissue engineering scaffold material. The electrospun natural polymer fiber membrane, which is porous, porosity adaptable and biocompatible,has great application in the tissue engineering scaffold material field.The using of silk-based biomaterials in applications of blood vessel repair, the medicine transmission carrier, the flesh wound to spread governs and so on, have been reported. Gelatin has the water affinity, it must be able to use after crosslinking.EDC/NHS is one kind of water-soluble crosslinking agent. Because the crossing linking intermediary product and itself may dissolve in the water which will not introduce the toxie ingredient in the crossing linking matrix, it was used as one kind of ideal mix biology crosslinking agent. But as a result of the gelatin water affinity, the water introduction in the crossinglink environment can create the gelatin nanofiber mat fierce contraetion,the curl,and affects the cross-linkage reaction advance which will not easily get crosslinked gelatin nanoflber mat flatly, smoothly and high degree o f crosslinking.In this paper,we crosslinked BSF nanofiber mats and TSF nanofiber mats in different rate of aleohol/water solutions with the different concentration EDC/NHS. According to the analysison their macroscopic performance, microcosmic performance, swelling, dissolution and mechanical properties, the search demonstrated that SF nanofiber mat which crosslinked in the absolute alcohol solution containing the best overall performance. We also made research on its behavior in vitro degradation process, and finally found the nanofiber mats may obtain the widespread application in the biological medical material domain after this kind of crossillnking. The research demonstrated that the fibroblast L373 and bone marrow mesenchymal stem cells (BMSCs) in mice can adhese and grow well on the natural crosslinked BSF and TSF nanofiber. So EDC/NHS-crosslinked BSF and TSF nanofiber mats were proved to be non-cytotoxic in vitro.