| ObjectiveTo investigate the effect of Bushen Antai Granule on recurrent spontaneous abortionmouse VEGF and VEGFR-2, RAS, MAPK, to investigate the treatment of Bushen Antai granule in recurrent abortion mechanism, for the clinical treatment of patients with recurrent spontaneous abortion of Chinese medicine to provide evidence,and lay the foundation for future animal experiments to further studyMethodsUsing Clark classic recurrent spontaneous abortion mouse model of DBA/ 2*CBA /J.Female CBA/J mice and male DBA/2 and BALB/c mice were mated by 2: 1, the establishment of RSA model of CBA/J * DBA/2 and CBA * BALB/c model of normal pregnancy, feeding detection pessary female rats for zeroth days of pregnancy meter.Female rats were divided into 6 groups, 10 rats in each group, the normal pregnant group, model control group were given distilled water; western medicine group was treated with progesterone capsules(0.4mg/kg.d) administration of traditional Chinese medicine; high, medium and low dose group, respectively 3.9g/kg.d, 11.7g/kg.d,35.1g/kg.d, diluting with distilled water by gavage for 15 days, each group.24h after the last administration, the mice were killed, section uterine observation of embryo loss; expression was detected in the decidua of VEGF and VEGFR-2, RAS,MAPK by immunohistochemical.Results①All mice 24 h after the last administration, the mice were killed, conducting a postmortemfound that uterine model group is dark red or even black, embryo size is not uniform, some are bamboo like change, the bleeding point visible black cut open after the uterus, the implantation site some apparent narrowing of the embryo or have machine of the organization. Each group of uterine pale red, embryonic development is good, no obviousblood stasis. Group of normal uterine pregnancy was also light red,embryonic developmentis good, uniform size, bleeding obvious intrauterine no, nocongestion in the implantation site.②Compared with the normal group, the other groups the abortion rate increased significantly, the difference was statistically significant(P<0.05); compared with the model group, the other groups abortion rate decreased significantly, the difference was statistically significant(P<0.05), and low dose of Chinese medicine group and Western medicine group, high dose of Chinese Medicine group, Chinese medicine dose groups were compared, there were statistical significant differences(P<0.05), comparing the rate of the group of Western medicine, Chinese medicine high dose group, middle dose group of the three abortion, no significant difference(P>0.05).③Compared with the normal group, the other groups of VEGF expression decreased obviously, the difference was statistically significant(P<0.05); compared with the model group, the expression of VEGF increased significantly in other groups, the difference was statistically significant(P<0.05); compared with the western medicine group, no obvious difference between the expression of VEGF in Chinese traditional medicine dose group(P>0.05); high expression of traditional Chinese medicine dose group, low dose of VEGF than in traditional Chinese medicine, western medicine group increased significantly(P < 0.05).④Compared with the normal group, the other groups of VEGFR-2 expression decreased obviously, the difference was statistically significant(P < 0.05); compared with the model group, the expression of VEGFR-2 increased significantly in other groups, the difference was statistically significant(P<0.05); compared with the western medicine group, no significant differences in VEGFR-2 expression dose group(P>0.05); the expression oftraditional Chinese medicine in traditional Chinese medicine high dose group, low dose of VEGFR-2 than in traditional Chinese medicine,western medicine group increased significantly(P < 0.05).⑤Compared with the normal group, the other the RAS expression was significantly decreased, the difference was statistically significant(P < 0.05); compared with the model group, the expression of RAS increased significantly in other groups, the difference was statistically significant(P < 0.05); compared with the western medicine group, no obvious difference between the expression of RAS in Chinese traditional medicine dose group(P>0.05 traditional Chinese Medicine); the expression of RAS of high dose group, low dose than in traditional Chinese medicine, western medicine group increased significantly(P < 0.05).⑥Compared with the normal group, the other groups of MAPK expression decreased obviously, the difference was statistically significant(P < 0.05); compared with the model group, the other groups the expression of MAPK increased significantly, and has statistical significance(P<0.05); compared with the western medicine group, no significant differences in MAPK expression dose group in Chinese medicine group(P>0.05); traditional Chinese medicine MAPK expression was significantly increased in high dose group than in the traditional Chinese medicine, low dose, the western medicine group(P < 0.05).ConclusionBushen Antai granule can decrease the recurrent spontaneous abortion mouse abortion rate, can improve the content of VEGF, VEGFR-2 protein expression in recurrent abortion decidual tissue of mice, up regulate the expression level of VEGF signaling pathway downstream of RAS protein, MAPK protein, which play the role of prevention of recurrent spontaneous abortion. |
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