| ObjectiveThis topic through the acupuncture and moxibustion combined with acupoint sticking therapy forchronic lumbar muscle strain, clinical treatment and curative effect observation. Systematic study on chronic lumbar muscle strain, thus providing a new thought and method for the treatment of chronic lumbar muscle strain.Methods24ratswererandomly dividedintoblankgroup (0.2mLsaline acupoint injection), exercise group of 21 days (0.2 mL saline acupoint injection) and total saponins of Panax ginseng acupoint injection group (0.2mL oftotalsaponinofPanaxginseng acupointinjection) group of 3. Exceptthe rats in the blank group (normal diet), the rest of the group with high 60cm,55cm diameterplastic drums as rat swimming tank,50cm water depth, water temperature 33-2 DEG C, indications of training 2 days later, began formal experiments to test for third days. Everyacupoint injection (unilateral, Zusanli and Neiguan, alternating every other day) was administered once,1 hours after the injection, in addition to the rats in the blank group, the remaining rats swimming training 2 times a day, each time the free swimming 15min, 10min clearance, training for 21 consecutive days (experiment to twenty-third days). In order to ensure the quality of swimming training, ready to stick in the barrel drive grilled pool wall edge or otherrats, the rest of the rat. In order to ensure the safety of rat training, and when the rats wereexhausted, then stop the swimming training. In the last movement, on the spontaneous activity ofbody weight, the number of rats, hippocampalultrastructure, apoptosis, hippocampalneuroncells in hippocampus neurotransmitter (NE,5-HT, DA, Ach, Glu, GABA) content were detected.Results1. compared with the control group, body weight of rats in the model group decreased (P<0.01), no significant differences in body mass of rats injected with body mass (P>0.05);compared with the model group, the body mass of acupoint injection group rats were increased significantly (P<0.01). Compared with the blank group, the model group rats and acupoint injection group rats were significantly reduced autonomic activities (both P<0.01); compared withthemodelgroup, acupoint injection group of independent activities of rats increased significantly (P<0.01).2. by toluidine blue staining, the hippocampal neurons of the rats in the blank group was neatcompact arranged, the interstitial cells and high density, uniform, mostly showed hyperchromaticnucleus shape, with clearly visible nucleoli, in addition, nerve cells, Nissl body is clearly visible, evenly distributed in the cytoplasm; whereas the hippocampal neurons of model group rats showed disorder loosely arranged like, around the cell gap widened, the uneven distribution ofcells, most cells showed incomplete structure shape, some cells pyknosis, irregular in shape, a single cell swelling cell body became larger and round, cytoplasm staining shallow and vague, nucleus condensation, the nucleolus is not very obvious, cells and Nissl body acupointdecreased; rats injected with pathological change is not obvious, the arrangement of single neuron, neuron structure are similar with the blank group.3. compared with the blank group, model group, acupoint injection group rat hippocampusapoptosis cells (positive cells number and the positive area ratio) increased significantly (bothP<0.01); compared with the model group, the apoptosis of hippocampal cells in rats of acupoint injection group (positive cell number and the positive area reduction ratio) were significant (bothP<0.01).4. compared with the blank group, the NE content of hippocampus in rats of model groupincreased significantly (P<0.01), no significant difference of NE content in rats with acupointinjection group (P>0.05); compared with the model group, the NE content of hippocampus in rats with acupoint injection group was significantly decreased (P<0.01). Compared with the blankgroup, the 5-HT content of hippocampusin ratsofmodelgroup increasedsignificantly (P<0.01), no significant difference of 5-HT content in rats with acupoint injection group (P>0.05);compared with the model group, the 5-HT content of hippocampus in rats with acupoint injectiongroup was significantly decreased (P<0.01). Compared with the blank group, the model grouprats and acupoint injection group rats were significantly lower DA content (both P<0.01);compared with the model group, the content of DA increased significantly in rat acupointinjection group (P <0.01); compared with the blank group, the rats in the model group andacupuncture point injection group, Ach content in rats significantly decreased (all P<0.01);compared with the model group, the content of Ach increased significantly in rat acupointinjection group (P<0.01).5. compared with the blank group, the Glu content of hippocampus in rats of model groupincreased significantly (P<0.01), no significant difference of Glu content in rats with acupointinjection group (P>0.05); compared with the model group, the Glu content of hippocampus inrats with acupoint injection group was significantly decreased (P< 0.01). Compared with the blank group, the model group and the acupuncture point injection group of rat hippocampal GABA content were increased significantly (P<0.01); compared with the model group, the GABA content of hippocampus in rats with acupoint injection group was significantly decreased(P<0.01). Compared with the blank group, the hippocampal GABA/Glu in the rats of model group increased significantly (P<0.01), no significant difference of acupoint injection in rats of GABA/Glu group (P>0.05); compared with the model group, hippocampal GABA/Glu acupoint injection group rats decreased significantly (P<0.01).Conelusion1. to "high 60cm, diameter 55cm plastic drums as swimming rats groove depth of 50cm, the water temperature of 33 plus or minus 2 DEG C, the rats swimming training 2 times a day, each time the free swimming 15min, gap 10min, training for 21 days in a row, in the swimming training in rats, with a wooden barrel drive PA in the pool the wall edge or other rats, the rest of the rat, and when rats were exhausted, then stop the swimming training" as the modeling methods, cancopy the successful exercise fatigue animal (rat) model.2. acupoint injection of ginseng saponins, can achieve the anti fatigue effect, can make the bodyquality of exercise-induced fatigue rats increased, autonomic activities significantly increased, hippocampal neurons of rats with exercise induced fatigue damage has been significantlyreversed arrangement, a single neuron, neuron structure were improved, the sports fatigue of apoptotic cells in hippocampus of rats and the number of positive area significantly lower than.The possible mechanism of anti fatigue 3, acupoint injection of ginseng total saponins is, can reduce the exercise-induced fatigue in rats hippocampus NE,5-HT, Glu and GABA content, increased the DA and Ach content, synthesis metabolism disorder reversal of hippocampusrelated neurotransmitters, recovery of excitation and inhibition of hippocampal function dynamic balance. |
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