Anti-hepatoma Effect And Mechanism Of The Essentialoil From Amomum Tsao-ko

Objective: To observe the anti-tumor effect of the essential oil from Amomum tsao-ko on H22 bearing mice in vivo and to explore its mechanism, To discuss geraniol combined with 4-propylbenzaldehyde of HepG2 cell proliferation inhibition of liver cancer, to explore the mechanism of action of essential oil from Amomum tsao-ko resistance of liver cancer. Methods: The anti-tumor effects of essential oil from Amomum tsao-ko(EOAT) in vivo was tested with the H22 tumor bearing mice model. The model mice were divided into five groups: high-, middle- and low-dose(75、150、300 mg·kg-1) EOAT groups, and 0.5% CMC-Na Negative control group and CTX group(Positive control, 20 mg·kg-1), The tissues of tumor, thymus, as well as spleen were dissected and weighted; The tumor inhibition rate, thymus index and spleen index were calculated. The morphological changes of tumor cells were observed by HE staining. The expression of apoptosis related protein Bax and Bcl-2 was detected using immunohistochemistry. Determined by MTT method is used to detect different concentrations of geraniol and 4-Propylbenzaldehyde separate and synergy effect on HepG2 liver cancer cell proliferation, Individual and collaborative drug inhibition rate and calculated index Q, observe the synergy effect. Results: All doses of EOAT significantly inhibit tumor growth on H22 mice(p<0.05), EOAT had a dose dependent relationship for the anti-tumor activity. However, the high dose of EOAT has slightly side-effects. The thymus and spleen indexes of all EOAT groups are all significantly increased(P<0.05) compared to those of CTX group. EOAT could increase the expression of Bax, reduce the expression of Bcl-2 In a conclusion, EOAT could inhibit the growth of H22 tumor cell. The mechanism might be related to raise the expression of Bax and suppress the expression of Bcl-2 to induce apoptosis. Different concentrations of geraniol with 4-Propylbenzaldehyde groups were significantly inhibited the proliferation of HepG2 cells in a concentration dependent manner, resulted in significant synergism. The number of HepG2 cells in combination therapy groups was reduced more significantly than those of geraniol, 4-propylbenzaldehyde alone groups and the change of cellular morphology was larger. Conclusions: EOAT had obvious anti-tumor effect on H22 tumor bearing mice. The mechanism might be related to raise the expression of Bax and suppress the expression of Bcl-2 to induce apoptosis. Geraniol and 4-propylbenzaldehyde alone had a certain inhibitory activity on HepG2 cell. The combination therapy of geraniol with 4-propylbenzaldehyde had the synergistic effect on HepG2 cell, and showed more obvious anti-hepatoma effect.