To Experimental Research On Intraperitoneal Injection Of Saturated Hydrogen Saline For Treating Traumatic Arthritis In Rabbis

Objective: inflammatory response is an important part in pathological change of traumatic arthritis(TA). Studies show that hydrogen has antioxidate and anti-inflammatory properties, however, there are few reports on treating TA with it. So this research aimed to investigate curative effects of curing traumatic arthritis in rabbits with intraperitoneal injection of saturated hydrogen saline solution. Methods: A total of 26 New Zealand white rabbits were divided into three groups: normal control group(n=6), model experimental group(n=10), and model control group(n=10). Rabbits in model experimental group were intraperitoneally injected with 8 ml/Kg saturated hydrogen saline, once every three day; equivalent saline was injected in rabbits of model control group;while no treatment was done on normal control group. The treatment lasted for 6weeks. Before and after the treatment, the serum and joint fluid of the rabbits in the three groups were respectively taken to determine the content of nitric oxide(NO) and hyaluronic acid(HA), which was used to evaluate therapeutic effect in combination with the behavioral performance of the rabbits. Results: Significant improvement in behavior was found in model experimental group after the treatment(P < 0.05). No statistical differences were found in knee swelling scores of the three groups before the treatment(P > 0.05), but model experimental group was superior to model control group in knee swelling scores after the treatment(P<0.05). The contents of NO both in serum( [79.58±13.46]vs [76.23±12.15]), and joint fluid( [89.45±14.98] vs [80.36±12.78]) in two model group increased before the treatment, which was of statistical difference(P< 0.05), but the NO content decreased dramatically in model experimental group after the treatment [(436.82±60.91) vs(340.21±40.57),P<0.05], and no obvious changes were observed in model control group(P > 0.05). The content of serum HA in model experimental group declined dramatically( P<0.05) and the joint fluid increased after the treatment( [1.72±0.37] vs [2.47±0.62],P <0.05), which no significant changes were found in model control group before and after the treatment. Conclusions: The intraperitoneal injection of saturated hydrogen saline may be effective in the treatment of traumatic arthritis.