Protection Of Sesame Oil On Peroxidation Injure Included By D-galactose

The body can produce free radicals in the metabolism processes while they can also be removed by free radical scavenger. Normally, the rate of producing and scavenging free radicals in our body can keep a dynamic balance.Once this balance is destroyed, the excess free radicals can damage normal biological functions and cause diseases such as cardiovascular and cerebrovascular diseases, caducity and tumor.The body can maintain stable free radical level through free radical scavengers. Edible oils is the necessary source of heat in people’s daily life, it also can provide essential fatty acids.So the study of how the functional oil improve our body’s state has became research focus in recent years. Sesame oil contain lipid, protein, vitamins and other nutrients as well as the unique sesame lignans, sesame lignans have antioxidative activity in vitro,it also have the functions of preventing lipid peroxidation and other functions.In this paper, using Hep G2 cells as experimental material and D-galactos as inducer, we established the model of oxidative stress of Hep G2 cells were inducing by D-gal, we also researched the protective effect of sesamin, sesamol on oxidative damage in Hep G2 cell. Taking D-gal as the inducer, using the following four concentrations of 37.5 m M, 75 m M,150 m M and 300 m M to induce Hep G2 cells, this research evaluated the effect of modeling by detecting the cell survival rateand determing content of MDA, SOD, GSH-PX in cells.The experimental results showed that: every concentration of D-gal group could cause oxidative damage to Hep G2 cells. Cells were in the proliferation stage in primary 24 h while were tending to the platform stage duing to the consumption of nutriention for growth closing to 72 h. MDA content increased significantly while SOD, GSH-PX content decrease significantly in group of 48 h. Combining with the survival rate of cells, we choosed the concentration is 75 m M, inducing 48 h to get the best model.The oil will be gradually oxidatived during storage, and then it can destruct the flavor of food and damage people’s health. Theoretically, sesame oil is not easy to maintain its quality during storage because it contains large amounts of unsaturated fatty acids. However, sesame oil can be storage inlong-term anditisnot easytobe oxidatived.Containing rich lignans in sesame oil endue its favourable antioxidant ability. In this experiment, accelerated oxidation method were used to study the scavenging efficiency changes of free radical and the content of lignans in sesame oil during storage. With the increase of storage time, lignan content decreased while clearing DPPH free radical, hydroxyl radicals also decreased, the decrease of antioxidant content resulted the decrease of antioxidant capacity.The experiment of scavenging free radical showed that: when concentration was 0.2mg/ml, sesamol’s scavenging efficiency of DPPH could reach 70% which higher than sesamin.When concentration of Sesamin was lower than 1.0mg/ml, the scavenging efficiency of DPPH was less than 30%. Sesamol’s scavenging efficiency of hydroxyl was slightly higher than that of sesamin.Cell experiments showed that: the contents of MDA were significantly decreased comparing with the model group while SOD, GSH-Px significantly increased in the group of sesamol. We also analysed the cell cycle through flow cytometry, the results showed that, the G1 phase of the cell ratio was significantly lower than that of model group, while the S phase ratio was significantly higher than the model group by adding sesamin and sesamol to cells.Both of results suggested that sesamin and sesamol caould alleviate Hep G2 oxidative damage induced by D-gal in a certain extent. When the sesamin concentration was 100 m M, the contents of MDA, SOD, GSH-PX in cells was respectively 32.41 ± 1.45 nmol/ml, 157.28 ± 7.31 U/mgprot, 31.36± 2.10 U/mgprot. When the sesamol concentration was 100 m M, the contents of MDA, SOD, GSH-PX in cells was respectively 15.82 ± 1.39238 nmol/ml, 28 ± 15.61238 U/mgprot, 28±15.61 U.Adding both sesame lignans to the cells, the contents of MDA in sesamol treatment groups were lower while the contents of SOD, high GSH-PX were higer than the sesamol treatment groups. Therefore, the protective effect on oxidative damage of cell with the same concentration of sesamol were better than that of sesamin. These results showed that the invitro antioxidant ability of sesamol were better than sesamin. In experiment we also got sesame oil emulsion of 5%, 10%,the results showed that 10% sesame oil emulsion can protect oxidative damaged Hep G2 cells which induced by D-gal.