Evaluation Of Notch1 And Wnt3a Signal Molecules In The Site Preservation With Autologous Bone Marrow Stem Cells-Platelet-Rich Fibrin Composite

Objective: We have explored the expression and significance of Notch and Wnt signaling pathway in the alveolar bone defect repaired process through the establ-ishment of animal models of autologous bone marrow mesenchymal stem cells composite platelet rich fibrin and repairing bone defects in rabbits extraction sockets.Method: Thirty-six male New Zealand white rabbits with two months old were randomly divided into four groups, and the left mandibular incisors of all rabbits w-ere removed by minimally invasive surgery under general anesthesia. Autologous b-one marrow mesenchymal stem cells platelet rich fibrin composites, single plateletrich fibrin and autologous bone marrow mesenchymal stem cells were implanted i n group A, B and C separately, and any material was not in the group D(blank c o-ntrol). Three rabbits from each group were killed at 4, 8 and 12 weeks after sur g-ery. Later, the bone defect was immediately drawn to make bone specimens. Theexpressions of Notch1 and Wnt3 a in the bone defect repair process were measure d by using immunohistochemical and immunofluorescence detection. Results: Immu n-ohistochemistry showed that the expressions of Notch1 and Wnt3 a in group A, B,and C were higher than that in group D at the fourth and eighth week after oper-ation(P<0.05). By contrast, the expressions of Notch1 and Wnt3 a in group D wer-e higher than that in group A, B, and C at the twelfth week(P<0.05). Immunoflu-orescence showed that both Notch1 and Wnt3 a expression reached their peaks in new bone cells of bone defect at the fourth week after surgery, and gradually decr-eased until the bone was repaired completely. Conclusion: Notch1 and Wnt3 a sign-aling molecules both expressed in the process of autologous bone marrow mesenc-hymal stem cells-platelet rich fibrin composite repairing the bone defect, so that they can accelerate the healing by adjusting the proliferation and differentiation of au-tologous bone marrow mesenchymal stem cells. Moreover, Notch1 and Wnt3 a expr-essed similarly. Therefore, crosstalk between both may exist.