The Study Of Chemerin In Children With Respiratory Syncytial Virus Pneumonia

ObjectiveTo investigate the clinical significance of chemerin in children with Respiratory syncytial virus (RSV) Pneumonia. The peripheral serum chemerin level as well as both proinflammatory factor:Tumor necrosis factor alpha (TNF-α), Interleukin 17 (IL-17) and anti-inflammatory factor:Interleukin 10 (IL-10), Transforming growth factor beta (TGF-β) were measured and their correlationship was analyzed.MethodsFrom October 2012 to April 2013,82 children with RSV Pneumonia from Children’s Hospital of Fudan University were recuited, including 65 mild cases and 17 severe. Forty children with common viral pneumonia and 40 normal children at the same time were enrolled as the control group. The concentration of chemerin, TNF-α, IL-17, IL-10 and TGF-β were determined by ELISA from all the children within 24 hours after admission. All statistic analysis was performed by SPSS ver 17.0.Results1. Relationship between chemerin and pneumonia severity:The serum level of chemerin from children with mild RSV Pneumonia were significantly higher than those from the healthy group (539.98±65.86 vs 337.24±43.37, P<0.01, respectively.). No difference existed in serum levels of chemerin between the cases with RSV Pneumonia and other viral pneumonia (539.98±65.86 vs 562.54±81.35, P >0.05, respectively.). The serum level of chemerin from children with severe RSV Pneumonia were significantly higher than those from the mild cases (786.62±82.59 vs 539.98±65.86, P<0.01, respectively.).2. Relationship between chemerin and proinflammatory factor(1) Comparion of TNF-α among groups:Serum level of TNF-α from children with mild RSV Pneumonia were significantly higher than those other viral pneumonia and the healthy group (33.14±3.00 vs 26.98±2.64 vs 19.74±3.49, P<0.01, respectively.); The serum level of TNF-α from children with severe RSV Pneumonia were significantly higher than those from the mild cases (55.28±4.47 vs 33.14± 3.00, P<0.01, respectively.).(2) Comparion of IL-17 among groups:Serum level of IL-17 from children with mild RSV Pneumonia were significantly higher than those other viral pneumonia and the healthy group (32.82±4.16 vs 26.98±4.21 vs 21.56±3.85, P<0.01, respectively.). The serum level of IL-17 from children with severe RSV Pneumonia were significantly higher than those from the mild cases (35.88±3.39 vs 32.82± 4.16, P< 0.01, respectively.).(3) The levels of chemerin and TNF-α & IL-17 (r=0.81, r=0.61, P<0.01, respectively.)were positive correlated.3. Relationship between chemerin and anti-inflammatory factor(1) Comparion of IL-10 among groups:Serum level of IL-10 from children with mild RSV Pneumonia were significantly lower than those other viral pneumonia and the healthy group (26.56±5.31 vs 39.37±11.68 vs 37.76±10.21, P<0.01, respectively.). The serum level of IL-10 from children with severe RSV Pneumonia were significantly lower than those from the mild cases (18.92±4.26 vs 26.56± 5.31, P< 0.01, respectively.).(2) Comparion of TGF-β among groups:Serum level of TGF- β from children with mild RSV Pneumonia were significantly lower than those other viral pneumonia and the healthy group (29.71±5.07 vs 50.29±12.90 vs 48.18±15.98, P<0.01, respectively.). The serum level of TGF-β from children with severe RSV Pneumonia were significantly lower than those in the mild cases (19.01±2.82 vs 29.71±5.07, P<0.01, respectively.).(3) The levels of chemerin and IL-10 & TGF-β (r=-0.80; r=-0.75,P<0.01, respectively.)were negative correlated.ConclusionThe serum chemerin levels elevated in children with RSV Pneuminia are associated with inflammatory severity and positive correlated to TNF-α & IL-17 while negative correlated to IL-10 & TGF-β.Chemerin plays an important role in pathogenesis of RSV Pneumonia in children.