Effect Of ω-3PUFA On The Inflammatory Mediator And P38 MAPK In Rats With Acute Pancreatitis

ObjectiveTo investigate effects of co-3PUFA on the inflammatory mediator and p38 MAPK in rats with acute pancreatitis.Methods150 Sprague Dawley rats, weighing 220～260g, with a 3.5% taurocholic acid (60ul/ min) induced low pressure retrograde injection of bilepancreatic duct model of the induction of SAP. After successful modeling, they were randomly divided into a control group of severe acute pancreatitis (SAP group), enteral nutrition group (EN group) and added ω-3PUFA enteral nutrition group (ω-EN group) 50 each group. In the first, fouth, seventh and fourteenth day, rats were sacrificed:detection of serum amylase, using enzyme-linked immunosorbent assay (ELISA) to detect cytokines tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), the light microscope with hematoxylin-eosin staining for pathological evaluation of pancreatic tissue; Immunohistochemical detection of P38 MAPK in rat pancreatic tissue, and the survival rate was calculated at 7 and 14 days.ResultsSerum amylase of three groups rats were significantly increased, the first day was the peak, and then declined. In each period SAP group, serum TNF-α were significantly increased, but IL-10 reduced, The fourth day reached the peak, P38 MAPK expression of pancreatic tissue was also significantly increased; Compared with SAP group, serum TNF-α of EN group and ω-EN group decreased significantly, the difference was statistically significant (P<0.05), but IL-10 increased, the difference was statistically significant (P<0.05). Compared with EN group, serum TNF-α of co-EN group were significantly decreased in seventh day and fourteenth day, but IL-10 increased significantly, the difference was statistically significant (P< 0.05). In seventh day, SAP group of rats all died, EN group and ω-EN group survived; In fourteenth day, EN group survived four (survival rate 8%) ω-EN group survived seven (survival rate 14%)ConclusionsAdd omega-3 polyunsaturated fatty acids of enteral nutrition is beneficial to improve the survival rate of the rats with acute pancreatitis, reduce the serum amylase level, inhibit the secretion of inflammatory mediators of TNF-α, increase the secretion of the anti-inflammatory cytokine IL-10 concentration, and its mechanism of action may inhibit the expression of rat acute pancreatitis p38MAPK thereby reducing the release of inflammatory medium.