Study The Role Of Blood Purification Therapy In The Endoplasmic Reticulum Stress Signaling Pathways In Dogs With Multiple Organ Dysfuncition Syndrome

Objective:To investigate the expression of endoplasmic reticulum stress marker proteins JNK2 gene in dogs with multiple organ dysfuncition syndrome (MODS) treated with high-volume hemofiltrationtreatment(HVHF).Methods:Two-hit method was used to establish the dog MODS model. Twelve Beagle dogs were randomly divided into normal control group (n=6),MODS group (n=6) and HVHF group (n=6). HVHF was performed about 24 hours after LPS injection in MODS dogs of HVHF group. Specimens from blood samples before operation (T1),before LPS injection and 0h(T2),6h(T3),12h(T4),24h(T5) after LPS injected. In vivo tests:Detecting JNK2 mRNA level in liver, lung and kidney tissue. In vitro tests:Serum of two groups to induce human umbilical vein endothelial cells (HUVECs) In vitro to establish endoplasmic reticulum stress apoptosis models. Determining JNK2mRNA expression, protein expression and endothelial cell apoptosis rate before and after siRNA interference. Results: In vivo tests:JNK2mRNA expression of liver in HVHF group were higher than MODS group (P<0.01). JNK2mRNA expression of lung in HVHF group were was significantly lower than MODS group (P<0.01). In vitro tests:1. JNK2 gene expression:Compared with the T1 time, HVHF group was significantly lower in T3-T5 JNK2mRNA level, the difference was statistically significant (P<0.01). Compared with the siRNA interference gived before, after interference the two group of JNK2mRNA expression levels were significantly decreased, the difference was statistically significant (P<0.01);2. JNK protein expression:Compared with the T1 time, HVHF group was significantly lower in JNK protein expression at T3-T5, the difference was statistically significant (P<0.01) Compared with the MODS group, HVHF group was significantly lower in JNK protein expression at T3-T5, the difference was statistically significant (P<0.01);Compared with the siRNA interference gived before, two groups of JNK protein expression was significantly decreased after the disturbance, the difference was statistically significant (P P<0.01);3.Endothelial cell apoptosis rate:Compared with the T1 time, HVHF group was significantly lower in the endothelial cell apoptosis rate at T3-T5, the difference was statistically significant (P<0.01); Compared with the MODS group, HVHF group was significantly lower in the endothelial cell apoptosis rate at T3-T5, the difference was statistically significant (P<0.01); Compared with the siRNA interference gived before, after interference the two groups were significantly decreased in the endothelial cell apoptosis rate, the difference was statistically significant (P<0.01). Conclusion: JNK signaling pathway mediated apoptosis in MODS dogs over endoplasmic reticulum stress, HVHF can reduced apoptosis by clearing inflammatory mediators, reducing oxidative stress, and reducing the endoplasmic reticulum stress.