| Objective: The present study is intended to determine the normal reference range of the 33 trace elements(Li, Be, B, Mg, Al, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga,As, Se, Rb, Sr, Zr, Mo, Ag, Cd, Sb, Cs, Ba, Au, Hg, Tl, Pb, Th and U) in human whole blood of general population in Hunan and investigate the content difference of age and sex on trace elements. Determining the normal reference range of the 33 trace elements of the population of Xinyu, Jiangxi,and comparisoning the population within the area of influence of mine spill.The study results will provide support for developing normal value of blood samples inorganic element database in Chinese population, the provided concentration ranges are also useful for further forensic toxicological analysis or other groups planning and conducting research, the provided concentration ranges are intended are useful for further forensic toxicological analysis or other groups planning and conducting research.Method:1 Collecting 1168 healthy subjects living in Yueyang, Zhangjiajie, Zhuzhou,Shaoyang of Hunan province and 166 healthy subjects living in the area of Xinyu,Jiangxi.2 The blood samples were disposed by a wet digestion method and analysed by inductively coupled plasma mass spectrometry(ICP-MS) for all 33 elements.3 The statistical calculations were porformed with SPSS software v.20.0 and thenormal reference range discribed by P5-P95.Method:1 The normal reference ranges of trace elements in whole blood is Li(0.47-87.6ng/m L),Be(<0.01-0.94ng/m L),B(70.2-229ng/m L),Mg(33128-50222ng/m L),Al(0.5-465ng/m L),Ca(45778-75844ng/m L),Ti(105-292ng/m L),V(0.66-4.55ng/m L),Cr(3.30-25.2ng/m L),Mn(8.54-28.0ng/m L),Fe(372428-564220ng/m L),Co(<0.005-0.89ng/m L),Ni(8.89-36.4ng/m L),Cu(664-1046ng/m L),Zn(6324-22586ng/m L), Ga(0.04-16.3ng/m L),As(2.17-14.5ng/m L),Se(171-310ng/m L),Rb(2123-4463ng/m L),Sr(11.9-52.3ng/m L),Zr(0.01-2.45ng/m L),Mo(0.48-4.02ng/m L),Ag(<0.005-1.63ng/m L),Cd(0.70-11.3ng/m L),Sb(<0.01-29.5ng/m L),Cs(3.30-12.0ng/m L),Ba(2.46-218ng/m L),Au(<0.01ng/m L),Hg(1.41-7.93ng/m L),Tl(<0.001-0.50ng/m L),Pb(22.5-126ng/m L),Th(<0.001-0.25ng/m L),U(<0.001-0.09ng/m L).2 There is statistical difference between men and women about the content of B,Mg, Ca, Ti, Mn, Fe, Co, Cu, Zn, As, Se, Rb, Sr, Ag, Cd, Cs, Hg and Pb.Men had higher levels of B, Mg, Ti, Fe, Zn, As, Se, Rb, Sr, Ag, Cd, Cs, Hg and Pb, while Ca, Mn, Co and Cu in blood was higher in women.3 Age was shown to influence the concentrations of Co, Ni, Cs and Hg in women and Cu, Se and Hg in men. Aged 40-59, the levels of Cs and Hg in women and Cu, Se and Hg in men were considered higher while Co and Ni in women were higher of aged20-39.4 There is statistical difference of the content of all elements came from four different regions of Hunan province.5 Concentrations of Ti, V, Fe, Cu and Pb studied were statistically higher in the population of the Xinyu mine spill with respect to that of the non-mine spill area in Zhuzhou.Conclusion:The normal reference values of 33 trace elements in whole blood of generalpopulation in Hunan were determined. Men had statistically higher levels of B, Mg, Ti,Fe, Zn, As, Se, Rb, Sr, Ag, Cd, Cs, Hg and Pb, while women had statistically higher levels of Ca, Mn, Co and Cu. Blood levels of Cs and Hg in women and Cu, Se and Hg in men were increased with age, while Co and Ni in women were decreased with aged.The availability of accurate trace element reference values in human tissues represents an important indicator to the health status of the general population and provides scientific evidence for forensic investigations. Normal reference values of 33 trace elements in blood were determined of living the affected by Xinyu mine spill,and the comparison of concentrations of Ti, V, Fe, Cu and Pb studied were statistically higher,maybe indicate that the spill had any incidence on human health in the population living in the affected area. |
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