Research On Repair Effect And Mechanism Of Scald Ointment On Rat With Second Deep Degree Scald Wound

Objective To research the repair effect and mechanism of Scald Ointment on rat with second deep degree scald wound,Jing Wan-hong Ointment being used as a control.Methods There are seventy-five SD rats.Second deep degree scald were made in rats.They were randomly divided into model group,positive control group and Scald Ointment group. Jing Wan Hong Ointment and Scald Ointment were separately applied on rats’ wound of positive control group and Scald Ointment group once a day.Edema, scabby skin and scab skin peeling of the wound were observed at the 7th,14 th and 21 th day.The healing area was measured periodically to calculate the rate of healing at the 7th,14 th and 21 th day. Tissue was collected from the wound of each group at the 7th,14 th and 21 th day.The tissue was made into pathological sections and immunohistochemical sections. IL-1β and Hydroxyproline in tissue was measured at the 7th,14 th and 21 th day.Results The healing rates of Scald Ointment group and positive control group were significantly higher than model group(P<0.05). Pathological sections revealed that Scald Ointment group and positive control group was better than model group in the quality of wound healing. Immunohistochemical sections showed that the positive expression of EGF in Scald Ointment group and positive control group was higher than that in model group at the 7th,14 th,21th day(P<0.05). IL-1β in tissue in Scald Ointment group and positive control group was lower than that in model group at the 7th,14 th day(P<0.05). Hydroxyproline in tissue in Scald Ointment group and positive control group was higher than that in model group at the 7th,14 th,21th day.(P<0.05).Conclusions Scald Ointment has the repair effect on rat with Second Deep Degree Scald wound.Its mechanism is possibly related to that Scald Ointment can reduce IL-1β in tissue and increase positive expression of EGF and Hyp in wound.