| Objective To detect the expression of Rho C and Fox M1 m RNA in peripheral blood of healthy people and patients with hepatocellular carcinoma(HCC), and to investigate whether it can be used as a new marker which hint diagnosis and staging of HCC. Methods The expression of Rho C and Fox M1 genes in peripheral blood was detected by reverse transcription polymerase chain reaction(RT-PCR) in 40 cases of hepatocellular carcinoma patients with extrahepatic metastasis within 1 year(group 1) and 40 cases of patients with no extrahepatic metastasis(group 2) and 20 cases of healthy adults(control group). Relative amount of m RNA of Rho C and Fox M1 m RNA was detected by semi-quantitative RT-PCR. Results There was no expression of Rho C and Fox M1 in control group, and the positive rate of Rho C m RNA was 67.5%(27/40) and 40.0%(16/40) respectively in group 1 and group 2, and differencewas statistically significant(x2 = 6.084, P = 0.014), and the positive rate of Fox M1 gene was 42.5%(17/40) and 20%(8/40) respectively in group 1 and group 2, and difference was statistically significant(x2 = 4.713, P = 0.030). The positive expression rate of Rho C/Fox M1 were increased by combinded detect in positive and negative extrahepatic metastatic HCC and the positive rate were respectively 80.0% and 42.5%. The expression levels of Rho C and Fox M1 in peripheral blood were significantly higher in patients with extrahepatic metastases than with no extrahepatic metastatic HCC(Rho C m RNA/beta actin: 0.61 ±0.21 vs 0.44± 0.12, t = 2.934, P = 0.005; Fox M1 m RNA/beta actin: 0.53±0.16 vs 0.28±0.07, t = 4.172, P = 0.000). Conclusion(1)There was no Rho C and Fox M1 expression in normal peripheral blood.(2)The expression of Rho C and Fox M1 in peripheral blood of patients with extrahepatic metastases was significantly higher than patients with no extrahepatic metastases.(3)The positive rate of expression of Rho C and Fox M1 in peripheral blood of patients with HCC elevated by combinded detect. |
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